DISTRIBUTION OF THE SEROTONIN 5-HT2 RECEPTOR FAMILY MESSENGER-RNAS - COMPARISON BETWEEN 5-HT(2A) AND 5-HT(2C) RECEPTORS
DISTRIBUTION OF THE SEROTONIN 5-HT2 RECEPTOR FAMILY MESSENGER-RNAS - COMPARISON BETWEEN 5-HT(2A) AND 5-HT(2C) RECEPTORS
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DOI：
10.1016/0169-328x(94)90223-2
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发表时间：
1994-04-01
期刊：
影响因子：
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通讯作者：
MENGOD, G
中科院分区：
文献类型：
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作者：
POMPEIANO, M;PALACIOS, JM;MENGOD, G
由于它们的相似性，5-HT2, 5-HT1C和最近描述的5-HT2F受体被归类为5-HT2受体家族的成员，它们分别被重新命名为5-HT2A， 5-HT2C和5-HT2B。采用原位杂交组织化学方法对大鼠脑连续组织切片5-HT2受体家族成员mRNA编码的区域分布和细胞定位进行了研究。未发现5-HT2B受体表达的证据。5-HT2A（原5-HT2）受体mRNA仅在少数区域高水平表达，如额叶皮质、梨状皮质、CA3的腹尾部、内侧乳核、脑干的脑桥核和颅运动神经核以及脊髓前角。5-HT2A受体mRNA的分布通常与相应结合位点的分布一致，尽管有时也会观察到差异。5-HT2C（原5-HT1C） mRNA在脉络膜丛中含量非常高。然而，在许多其他脑区，如脾后皮质、梨状皮质和内嗅皮质、前嗅核、隔外侧核、丘脑下核、杏仁核、CA3的下背和腹侧部分、外侧缰核、黑质致密部、几个脑干核和整个脊髓灰质中也可见到非常高的水平。这些结果证实并扩展了先前的观察结果，即5-HT2C受体mRNA存在于许多大脑区域，除了放射自显像显示具有相应的结合位点，并且5-HT2C受体亚型是大脑中主要的5-HT受体。从它们的分布比较来看，5-HT2A和5-HT2C受体mrna似乎在不同但重叠的大脑区域中表达。这两种mrna在前嗅核、梨状皮质、梨状内核、屏状体、CA3腹侧锥体细胞层、带绦虫、致密黑质和几个脑干核中高水平共存。在其他区域，这两种mrna都存在，但分布不同，如尾核-壳核。这些结果也讨论了在大脑中存在的两个如此相似的受体亚型的生理意义。
Because of their similarities, serotonin 5-HT2, 5-HT1C, and the recently described 5-HT2F receptors have been classified as members of the 5-HT2 receptor family, and they have been renamed 5-HT2A, 5-HT2C and 5-HT2B, respectively. The regional distribution and cellular localization of mRNA coding for the members of 5-HT2 receptor family were investigated in consecutive tissue sections from the rat brain by in situ hybridization histochemistry. No evidence for the expression of 5-HT2B receptor was found. High levels of 5-HT2A (formerly 5-HT2) receptor mRNA were observed only in few areas, as the frontal cortex, piriform cortex, ventro-caudal part of CA3, medial mammilary nucleus, the pontine nuclei and the motor cranial nerve nuclei in the brainstem, and the ventral horn of the spinal Cord. The distribution of 5-HT2A receptor mRNA is generally in good agreement with that of the corresponding binding sites, although discrepancies were sometimes observed. 5-HT2C (formerly 5-HT1C) mRNA was present at very high levels in the choroid plexuses. However, very high levels were also seen in many other brain regions, as the retrosplenial, piriform and entorhinal cortex, anterior olfactory nucleus, lateral septal nucleus, subthalamic nucleus, amygdala, subiculum and ventral part of CA3, lateral habenula, substantia nigra pars compacta, several brainstem nuclei and the whole grey matter of the spinal cord. These results confirm and extend previous observations that 5-HT2C receptor mRNA is present in many brain areas in addition to those autoradiographically shown to have the corresponding binding sites and that 5-HT2C receptor subtype is a principal 5-HT receptor in the brain. From the comparison between their distributions, 5-HT2A and 5-HT2C receptor mRNAs appeared to be expressed in distinct but overlapping sets of brain regions. Both mRNAs coexisted at high levels in the anterior olfactory nucleus, piriform cortex, endopiriform nucleus, claustrum, pyramidal cell layer of the ventral part of CA3, taenia tecta, substantia nigra pars compacta, and several brainstem nuclei. In other regions both mRNAs were present but with different distributions, as the caudate-putamen. These results are also discussed in relation to the physiological meaning of the existence of two so similar receptor subtypes in the brain.