Establishment and characterization of a diethylnitrosamine‐initiated woodchuck hepatocyte cell line
Establishment and characterization of a diethylnitrosamine‐initiated woodchuck hepatocyte cell line
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二乙基亚硝胺引发的土拨鼠肝细胞系的建立和表征
DOI：
10.1002/hep.1840070524
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发表时间：
1987
期刊：
影响因子：
13.5
通讯作者：
T. Kitagawa
中科院分区：
文献类型：
--
作者：
Gang‐hong Lee;T. Kawaguchi;K. Nomura;T. Kitagawa
将不含土拨鼠肝炎病毒的土拨鼠体内用二乙基亚硝胺处理2个月，然后将酶灌注获得的肝细胞与保肝剂苯巴比妥一起培养。这种体内-体外过程产生了增殖的上皮细胞灶，从其中一个细胞灶中建立了目前描述的肝细胞系（WLC-3）并对其进行了表征。
Woodchucks free from woodchuck hepatitis virus were treated with diethylnitrosamine in vivo for 2 months, and then hepatocytes obtained by enzymatic perfusion were cultured with the hepatopromoter phenobarbital. This in vivo‐in vitro procedure gave rise to proliferating epithelial cell foci, from one of which the presently described hepatocyte cell line (WLC‐3) was established and characterized.