Mg-Zn-Mn alloy extract induces the angiogenesis of human umbilical vein endothelial cells via FGF/FGFR signaling pathway
Mg-Zn-Mn alloy extract induces the angiogenesis of human umbilical vein endothelial cells via FGF/FGFR signaling pathway
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DOI：
10.1016/j.bbrc.2019.04.198
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发表时间：
2019-06-30
影响因子：
3.1
通讯作者：
Li, Aoyu
中科院分区：
文献类型：
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作者：
Li, Ding;Yuan, Qi;Li, Aoyu
镁及其合金作为一种生物可降解材料，具有良好的生物相容性、生物可降解性和与骨相似的力学性能，在体外可促进成骨细胞分化，在体内可诱导新骨形成，已被广泛应用于骨骼肌领域。本研究制备了Mg-Zn-Mn合金提取物，并研究了其对人脐静脉内皮细胞（HUVECs）血管生成的影响。本研究制备了不同浓度的Mg-Zn-Mn合金浸提液，并用这些浸提液培养HUVECs。6.25%镁合金浸提液能显著促进HUVECs的DNA合成能力、细胞活力和管腔形成能力。同时，6.25%镁合金提取物处理后p-FGFR/FGFR、p-PI 3 K/P13 K、p-AKT/AKT比值显著升高，而FGFR/FGFR信号通路抑制剂BFJ 398处理后则显著降低，表明6.25%镁合金提取物可通过激活FGF/FGFR信号通路促进HUVECs血管生成。总之，这些数据表明，6.25%的Mg-Zn-Mn合金提取物通过FGF信号通路诱导HUVECs的血管生成。需要进一步的体内实验来进一步证实目前的体外研究结果。(C)2019由Elsevier Inc.出版
Magnesium (Mg) and its alloys as a type of different biodegradable materials have been used in the musculoskeletal field because of their excellent biocompatibility, biodegradability and mechanical properties similar to bone; besides, Mg could promote osteoblast differentiation in vitro and induce the formation of new bone in vivo. In the present study, we prepared the extracts of Mg-Zn-Mn alloy and examined their effects on the angiogenesis of human umbilical vein endothelial cells (HUVECs). In the present study, we prepared Mg-Zn-Mn alloy extracts of different concentrations and cultured HUVECs with these extracts. The DNA synthesis capacity, the cell viability, and the tube formation capacity of HUVECs could be significantly induced by 6.25% Mg alloy extract. In the meantime, the ratios of p-FGFR/FGFR, p-PI3K/P13K, and p-AKT/AKT were significantly increased by 6.25% Mg alloy extract treatment, while decreased by FGFR/FGFR signaling pathway inhibitor BFJ398, indicating that 6.25% Mg alloy extract could promote the angiogenesis of HUVECs via activating FGF/FGFR signaling pathway. In conclusion, these data indicate that 6.25% Mg-Zn-Mn alloy extract induces the angiogenesis of HUVECs via FGF signaling pathway. Further in vivo experiments are needed to further confirm the present in vitro findings. (C) 2019 Published by Elsevier Inc.