FIBRONECTIN EXTRACELLULAR-MATRIX ASSEMBLY BY HUMAN EPIDERMAL-CELLS IMPLANTED INTO ATHYMIC MICE
FIBRONECTIN EXTRACELLULAR-MATRIX ASSEMBLY BY HUMAN EPIDERMAL-CELLS IMPLANTED INTO ATHYMIC MICE
复制标题
DOI：
10.1111/1523-1747.ep12460874
复制
发表时间：
1988-04-01
影响因子：
6.5
通讯作者：
CLARK, RAF
中科院分区：
文献类型：
--
作者：
GRIMWOOD, RE;BASKIN, JB;CLARK, RAF
人表皮角质形成细胞在胸腺小鼠皮下植入后重组成表皮包涵囊。在这些囊肿的组织和成熟过程中，纤维连接蛋白在周围的细胞外基质中积累，基底膜蛋白、大泡类天疱疮抗原和层粘连蛋白出现在上皮-基质界面。这些蛋白质出现的顺序与体内皮肤伤口再上皮化过程中所见的相似。纤维连接蛋白在表皮细胞聚集期间出现，并在囊肿周围区域持续存在至少7天。大疱性类天疱疮抗原和层粘连蛋白出现较晚（分别为4天和7天），并最终在囊肿周围组织成连续的带状。大疱性类天疱疮抗原和层粘连蛋白在基质-上皮界面的分布可持续至少5周，这表明移植的表皮细胞能够发育并维持稳定的基底膜区。纤维连接蛋白在囊肿组织和成熟过程中大量存在于表皮囊肿附近的基质和周围的基质中，在5周后减少到检测不到的水平。虽然大部分纤维连接蛋白来自宿主组织，但针对人类纤维连接蛋白的物种特异性抗体表明，至少有一部分这种蛋白是由植入的表皮细胞合成和沉积的。
Human epidermal keratinocytes reorganize into epidermal inclusion cysts when implanted subcutaneously into athymic mice. During the organization and maturation of these cysts, fibronectin accumulates in the surrounding extracellular matrix and the basement membrane proteins bullous pemphigoid antigen and laminin appear at the epithelial-stromal interface. The sequence in which these proteins appear parallels that seen during reepithelialization of a skin wound in vivo. Fibronectin appears during aggregation of the epidermal cells and persists in the area surrounding the cysts for at least 7 days. Bullous pemphigoid antigen and laminin appear later (by 4 and 7 days, respectively) and ultimately become organized into a continuous band at the periphery of the cyst. This distribution of bullous pemphigoid antigen and laminin at the stromal-epithelial interface persists at least 5 weeks, suggesting that the implanted epidermal cells are capable of developing and maintaining a stable basement membrane zone. Fibronectin, which is abundant in the matrix adjacent to the epidermal cysts and in the surrounding stroma during cyst organization and maturation, diminishes to undetectable levels by 5 weeks. While much of the fibronectin derives from the host tissues, species-specific antibodies to human fibronectin reveal that at least a portion of this protein is synthesized and deposited by the implanted epidermal cells.