AN IMMUNOELECTRON MICROSCOPIC AND IMMUNOFLUORESCENT STUDY ON THE ANTIGENIC RELATIONSHIP BETWEEN THE CORONAVIRUS-LIKE AGENT, CV-777, AND SEVERAL CORONAVIRUSES
AN IMMUNOELECTRON MICROSCOPIC AND IMMUNOFLUORESCENT STUDY ON THE ANTIGENIC RELATIONSHIP BETWEEN THE CORONAVIRUS-LIKE AGENT, CV-777, AND SEVERAL CORONAVIRUSES
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DOI：
10.1007/bf01315166
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发表时间：
1981-01-01
影响因子：
2.7
通讯作者：
REYNOLDS, DJ
中科院分区：
文献类型：
--
作者：
PENSAERT, MB;DEBOUCK, P;REYNOLDS, DJ
用免疫电子显微镜(IEM)和免疫荧光(IF)检测了猪肠道致病冠状病毒(CVLA)CV777与6种已知冠状病毒之间可能的抗原性关系。CVLA与传染性支气管炎病毒、传染性胃肠炎病毒(TGEV)、犬冠状病毒(CCV)、血凝性脑脊髓炎病毒(HEV)、新生小牛腹泻冠状病毒(NCDCV)、猫传染性腹膜炎病毒(FIPV)无交叉反应。IEM检测TGEV与CCV、NCDCV与HEV的抗原关系。用间接免疫荧光检测TGEV与CCV、TGEV与FIPV、HEV与NCDCV之间的抗原关系。
A possible antigenic relationship between the porcine enteropathogenic coronavirus-like agent (CVLA) CV777 and 6 known coronaviruses was examined by immunoelectron microscopy (IEM) and by immunofluorescence (IF). CVLA did not show cross reactivity with infectious bronchitis virus, transmissible gastroenteritis virus (TGEV), canine coronavirus (CCV), hemagglutinating encephalomyelitis virus (HEV), neonatal calf diarrhea coronavirus (NCDCV) or feline infectious peritonitis virus (FIPV). Antigenic relationship was detected by IEM between TGEV and CCV, and NCDCV and HEV. Antigenic relationship was detected by IF between TGEV and CCV, TGEV and FIPV, and HEV and NCDCV.