The induction of DNA puff BhC4-1 gene is a late response to the increase in 20-hydroxyecdysone titers in last instar dipteran larvae
The induction of DNA puff BhC4-1 gene is a late response to the increase in 20-hydroxyecdysone titers in last instar dipteran larvae
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DOI：
10.1016/s0925-4773(01)00589-5
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发表时间：
2002-01-01
影响因子：
2.6
通讯作者：
Paçó-Larson, ML
中科院分区：
文献类型：
--
作者：
Basso, LR;Vasconcelos, C;Paçó-Larson, ML
在携带BhC4-1基因的转基因果蝇中，扩展了BhC4-1基因表达的特征，并研究了其对20-羟基蜕皮激素的响应。在这两种生物中，唾液腺中BhC4-1的激活发生在幼虫期结束时，与诱导变态的蜕皮素滴度高峰相一致。注射20-羟基蜕皮素后，水螅4龄中期幼虫体内BhC4-1的表达、扩增和膨化C4的扩增是该激素诱导的后期事件。在20-羟基蜕皮素介导的转基因唾液腺中也观察到BhC4-1表达的晚期反应。利用转基因果蝇进行的体外研究表明，抑制因子和激活因子都能调控BhC4-1在唾液腺中的表达时间。(C) 2002爱思唯尔科学爱尔兰有限公司版权所有。
The characterization of DNA puff BhC4-1 expression was extended and its response to 20-hydroxyecdysone investigated in Bradysia hygida and in transgenic Drosophila carrying the BhC4-1 gene. In both organisms the activation of BhC4-1 in salivary glands occurs at the end of the larval stage coinciding with the peak in ecdysone titers which induces metamorphosis. Injections of 20-hydroxyecdysone into mid-fourth instar larvae of B. hygida show that the induction of BhC4-1 expression, as well as amplification and puff C4 expansion, are late events induced by the hormone. This late response of BhC4-1 expression was also observed in transgenic salivary glands cultivated in the presence of 20-hydroxyecdysone. In vitro studies using transgenic Drosophila indicate that both repressor and activator factors regulate the timing of BhC4-1 expression in salivary glands. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.