Effects of age and luteinizing hormone antiserum on human chorionic gonadotropin-stimulated testosterone secretion in young male rats.
Effects of age and luteinizing hormone antiserum on human chorionic gonadotropin-stimulated testosterone secretion in young male rats.
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年龄和黄体生成素抗血清对年轻雄性大鼠绒毛膜促性腺激素刺激的睾酮分泌的影响。
DOI：
10.1016/0024-3205(85)90411-4
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发表时间：
1985
期刊：
影响因子：
6.1
通讯作者：
Rikihisa,Y
中科院分区：
文献类型：
--
作者：
Lin,YC;Fukaya,T;Rikihisa,Y
研究了不同年龄的年轻雄性大鼠的类固醇生成能力。在5、10、15、20、25和30日龄时，各组大鼠在献祭前2 d肌肉注射人绒毛膜促性腺激素（HCG） 20 IU，每日2次/只，或黄体生成素（LH）抗血清0.25 ml，每日2次/只。对照组大鼠给予生理盐水或正常羊血清（NSS）。对照组大鼠血清睾酮和睾丸蛋白平均浓度分别为0.85±0.03 ng/ml和1.35±0.06 ng/mg。在第15天，hcg处理大鼠的血清和睾丸睾酮浓度分别显著增加到9.30±0.85 ng/ml和11.92 ng/mg睾丸蛋白。在同一年龄，同时给予LH/AS和HCG， HCG诱导的高水平血清和睾丸睾酮浓度显著降低至2.80±0.70 ng/ml和6.02±1.00 ng/mg蛋白。我们的研究结果表明，对HCG刺激的睾酮分泌与年龄有关。还确定，LH/ as处理大鼠的循环促性腺激素中和降低了间质细胞对促性腺激素刺激的敏感性。该体内模型将为研究发育中的年轻男性睾丸功能提供一个极好的机会。
The steroidogenic capacity of young male rats of different ages was studied. Two days prior to sacrifice at 5, 10, 15, 20, 25 and 30 days of age, the rats in treatment groups were given intramuscularly either human chorionic gonadotropin (HCG) at 20 IU twice daily/rat or luteinizing hormone (LH) antiserum (AS) at 0.25 ml twice daily/rat. Either saline or normal sheep serum (NSS) was given to control rats. The serum and testicular testosterone concentrations in the control rats averaged 0.85±0.03 ng/ml and 1.35±0.06 ng/mg testicular protein, respectively. At day-15 the serum and testicular testosterone concentrations in the HCG-treated rats had significantly increased to 9.30±0.85 ng/ml and 11.92 ng/mg of testicular protein, respectively. At the same age, the HCG-induced higher levels of serum and testicular testosterone concentrations were significantly reduced to 2.80±0.70 ng/ml and 6.02±1.00 ng/mg protein by concomitant administration of LH/AS and HCG. Our results suggest that the testosterone production in response to HCG stimulation is age-related. It was also determined that neutralization of circulating gonadotropin in LH/AS-treated rats decreased the sensitivity of Leydig cells to gonadotropin stimulation. This in vivo model should provide an excellent opportunity for the investigation of the testicular function in developing young males.