Fast manipulation of cellular cAMP level by light in vivo
Fast manipulation of cellular cAMP level by light in vivo
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DOI：
10.1038/nmeth975
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发表时间：
2007-01-01
期刊：
影响因子：
48
通讯作者：
Nagel, Georg
中科院分区：
文献类型：
--
作者：
Schroeder-Lang, Saskia;Schwaerzel, Martin;Nagel, Georg
纤细裸藻（Euglena gracilis）含有光活化腺苷酸环化酶（PAC），其由黄素蛋白PACa和PACb组成。在这里，我们报告的PAC在非洲爪蟾卵母细胞，HEK293细胞和果蝇，神经元表达产生光诱导的行为变化的功能表达。PAC的活性被蓝光强烈且可逆地增强，为动物细胞中cAMP的光诱导操纵提供了有力的工具。(c)2007年，自然出版集团。
The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACa and PACb. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells. (c) 2007 Nature Publishing Group.