Rapid fabrication of highly porous and biocompatible composite textile tubular scaffold for vascular tissue engineering
Rapid fabrication of highly porous and biocompatible composite textile tubular scaffold for vascular tissue engineering
复制标题
DOI：
10.1016/j.eurpolymj.2017.08.054
复制
发表时间：
2017-11-01
影响因子：
6
通讯作者：
Ivanovski, Saso
中科院分区：
文献类型：
--
作者：
Abdal-Hay, Abdalla;Memic, Adnan;Ivanovski, Saso
用于血管组织工程应用的三维 (3D) 结构需要具有高多孔结构、高生物相容性和机械稳定性的支架。本文采用喷气纺丝（AJS）纺织技术，同时逐层沉积由不同比例的聚（ε-己内酯）（PCL）和聚酰胺-6（PA-6）组成的复合纤维管状支架。具体来说，我们报告了复合多孔支架制造的最佳参数，这些参数可以精确控制一般支架结构以及支架的物理和机械性能。进行体外细胞培养研究，以研究聚合物成分和支架结构对 EA.hy926 人内皮细胞在制造的支架上粘附的影响。细胞培养结果表明，低 PA-6 纤维含量的复合支架是 EA.hy926 粘附和增殖最有希望的基质。基于目前的发现，这些高度多孔的复合管状结构支持内皮细胞迁移和细胞浸润，因此代表了用于血管组织工程的有前途的纳米纤维支架。
Three dimensional (3D) constructs for vascular tissue engineering applications require scaffolds with highly porous architectures, high biocompatibility and mechanical stability. In this paper, composite fibrous tubular scaffolds composed of different ratios of poly(epsilon-caprolactone) (PCL) and polyamide-6 (PA-6) were simultaneously deposited layer by layer by employing the air jet spinning (AJS) textile technique. Specifically, we report on the optimal parameters for the fabrication of composite porous scaffolds that allow for precise control over the general scaffold architecture, as well as the physical and mechanical properties of the scaffolds. In vitro cell culture study was performed to investigate the influence of polymer composition and scaffold architecture on the adhesion of EA.hy926 human endothelial cells onto the fabricated scaffolds. The cell culture results indicated that a composite scaffold with low PA-6 fibrous content is the most promising substrate for EA.hy926 adhesion and proliferation. Based on the present findings, these highly porous composite tubular constructs support endothelial cell migration and cellular infiltration, and hence represent promising nano-fibrous scaffolds for vascular tissue engineering.