DISSOCIATION OF INFLUENZA-VIRUS HEMAGGLUTININ AND NEURAMINIDASE ELIMINATES THEIR INTRAVIRIONIC ANTIGENIC-COMPETITION
DISSOCIATION OF INFLUENZA-VIRUS HEMAGGLUTININ AND NEURAMINIDASE ELIMINATES THEIR INTRAVIRIONIC ANTIGENIC-COMPETITION
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DOI：
10.1128/jvi.67.10.5721-5723.1993
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发表时间：
1993-10-01
影响因子：
5.4
通讯作者：
KILBOURNE, ED
中科院分区：
文献类型：
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作者：
JOHANSSON, BE;KILBOURNE, ED
当在完整的流感病毒颗粒上一起呈递时，外部血凝素（HA）和神经氨酸酶（NA）抗原是竞争性的，其中HA在T细胞和B细胞引发中均优于NA（B. E. Johansson，T. M. Moran和E. D.基尔本，美国国家科学院院刊Acad. Sci. USA 84：6869-6873，1987）。HA和NA从病毒中的解离和纯化以及它们单独或组合注射到BALB/c小鼠中消除了它们的抗原竞争，如通过抗体应答所测量的，这证实了它们的结构缔合导致了我们所称的病毒内抗原竞争。我们讨论这种现象与以前描述的分子间抗原竞争，并就其可能的机制。我们的研究结果是有关病毒疫苗载体和多组分疫苗的当代利益。
When presented together on the intact influenza virus particle, the external hemagglutinin (HA) and neuraminidase (NA) antigens are competitive, with HA dominant over NA in both T- and B-cell priming (B. E. Johansson, T. M. Moran, and E. D. Kilbourne, Proc. Natl. Acad. Sci. USA 84:6869-6873, 1987). Dissociation and purification of HA and NA from virus and their injection separately or in combination into BALB/c mice eliminates their antigenic competition as measured by antibody response, confirming that it is their structural association that leads to what we have termed intravirionic antigenic competition. We discuss this phenomenon with respect to previously described intermolecular antigenic competition and with regard to its probable mechanism. Our findings are relevant to contemporary interest in viral vaccine vectors and multicomponent vaccines.