Expression of candidate plasticity-related gene 15 is increased following traumatic brain injury
Expression of candidate plasticity-related gene 15 is increased following traumatic brain injury
复制标题
候选可塑性相关基因 15 的表达在创伤性脑损伤后增加
DOI：
10.1179/1743132812y.0000000134
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发表时间：
2013-03-01
影响因子：
1.9
通讯作者：
Fei, Zhou
中科院分区：
文献类型：
--
作者：
He, Yalong;Yang, Guitao;Fei, Zhou
【摘要】目的：候选可塑性相关基因15 （cpg15）是调节突触可塑性的活性调控基因；本研究评估了cpg15表达水平与创伤性脑损伤（TBI）后修复和再生之间的潜在联系。方法：采用免疫组化、Western blot、逆转录-聚合酶链反应等方法检测cpg15在脑外伤大鼠和假手术大鼠额叶中的表达。结果：CPG15+神经元在第1天出现在额叶冠状面，在第14天左右达到最高水平，并在第21天保持较高水平。CPG15蛋白和mRNA水平也以类似的时间模式升高。相比之下，假手术组大鼠的CPG15水平未检测到。结论：cpg15在额叶皮层的表达升高表明其可能参与脑外伤后的再生和修复过程。
Abstract Objectives: Candidate plasticity-related gene 15 (cpg15) is an activity-regulated gene that mediates synaptic plasticity; this study assesses the potential link between cpg15 expression levels and repair and regeneration following traumatic brain injury (TBI). Methods: We investigated the expression of cpg15 in the frontal lobe of rats subjected to TBI and sham rats, using the following methods: immunohistochemical analysis, Western blotting, and reverse transcription-polymerase chain reaction. Results: CPG15+ neurons were present in coronal sections of the frontal lobe at Day 1, reached the highest level around Day 14, and maintained elevated levels through Day 21. CPG15 protein and mRNA levels were noticed to increase in a similar temporal pattern. In contrast, rats in the sham group had undetectable levels of CPG15. Conclusions: Elevated cpg15 expression in the frontal cortex suggests its possible involvement in regenerative and reparative processes that follow TBI.