Allixin, a phytoalexin from garlic, inhibits the growth of Helicobacter pylori in vitro.
Allixin, a phytoalexin from garlic, inhibits the growth of Helicobacter pylori in vitro.
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大蒜素是一种来自大蒜的植物抗毒素，可在体外抑制幽门螺杆菌的生长。
DOI：
10.1111/j.1572-0241.2001.05351.x
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Pendland,SL
中科院分区：
文献类型：
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作者：
Mahady,GB;Matsuura,H;Pendland,SL
TO THE EDITOR: Gastric cancer currently remains the second most common cause of cancer-related deaths in the world, exceeded only by lung cancer (1). Although the etiology of gastric cancer is not well understood, there is compelling evidence to suggest that Helicobacter pylori (HP) infections are associated with gastric carcinoma, as well as primary gastric B-cell lymphoma, and HP has been classified as a group I carcinogen (1, 2). Interestingly, a high intake of vegetables in the garlic family has been associated with a reduction in the incidence of stomach cancer, and extracts of garlic have been reported to inhibit the growth of HP in vitro (3). However, in a recent clinical trial, administration of fresh garlic had no effect on HP infections and did not reduce urease activity as measured by a breath test (4). To address some of these inconsistencies we have investigated the susceptibility of HP to a concentrated garlic extract and allixin (3-hydroxy-5-methoxy-6-methyl-2-pentyl-4H-pyran-4-one), a phenolic phytoalexin found only in stressed garlic bulbs.A total of 15 clinical isolates and one American Type Culture Collection (ATCC 43504) of HP strains were used in the susceptibility testing, as previously described (5). A methanol extract of dried garlic bulbs (Allium sativum) was prepared and concentrated to dryness under reduced pressure, and a stock solution containing 10 mg/ml of dimethyl sulfoxide/water (1: 1) was used in the assay. A stock solution of pure allixin (10 mg/ml) in dimethyl sulfoxide/water (1: 1) was also assayed. The stock solutions of the garlic extract or pure allixin were added to the agar medium for final concentrations ranging from 6.25 μg to 100 μg/ml of agar. Vehicle solvent was added to the control cultures in the appropriate concentrations. The minimal inhibitory concentrations (MICs)(the minimal concentration of drug required to kill 99.9% of the bacterium) were determined. The meth-
