Optimal conditions for the growth of malignant human and animal cell populations in immunosuppressed mice.
Optimal conditions for the growth of malignant human and animal cell populations in immunosuppressed mice.
复制标题
免疫抑制小鼠中恶性人类和动物细胞群生长的最佳条件。
作者：
E. J. Stanbredge;L. Boulger;C. Franks;J. A. Garrett;D. Reeson;D. Bishop;F. Perkins
免疫抑制小鼠已被用于支持异种人和动物恶性细胞群的生长。肿瘤生长的最佳条件是新生儿胸腺切除术加上抗胸腺细胞血清或胸腺切除术，然后全身照射和骨髓重建。当小鼠接种正常和恶性细胞的混合物时，恶性细胞具有选择性优势。当混合种群在体外生长时，没有发现这种选择性。人类肿瘤也可以在免疫抑制小鼠中生长。这些肿瘤保留了人类宿主中原始肿瘤的组织。该系统的优点，癌症研究人员进行了讨论。
Immunosuppressed mice have been used to support the growth of xenogeneic human and animal malignant cell populations. The optimal conditions for tumor growth are neonatal thymectomy coupled with antithymocyte serum or thymectomy, followed by whole-body irradiation and bone marrow reconstitution. When mice are inoculated with a mixture of normal and malignant cells, the malignant cells have a selective advantage. No such selectivity is found when the mixed populations are grown in vitro. Human tumors may also be grown in immunosuppressed mice. These tumors retain the organization of the original tumor in the human host. The advantages of this system to cancer researchers are discussed.