Reconstitution of acetylcholine receptor function in lipid vesicles of defined composition.
Reconstitution of acetylcholine receptor function in lipid vesicles of defined composition.
复制标题
重建确定成分的脂质囊泡中的乙酰胆碱受体功能。
DOI：
10.1016/0005-2736(83)90379-6
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
McNamee,MG
中科院分区：
文献类型：
--
作者：
Ochoa,EL;Dalziel,AW;McNamee,MG
在由纯化的加利福尼亚电鳐乙酰胆碱受体和各种确定的脂质制备的重构膜中，研究了特定脂质对乙酰胆碱受体功能特性的影响。胆固醇和带负电荷的脂质大大增强了离子流入的囊泡的反应，作为测量的受体激动剂的阳离子易位的囊泡的效果。部分脂质依赖性效应可归因于囊泡平均大小的改变。所有使用的脂质混合物允许完全纳入受体和保留配体结合特性。离子通量特性的定量差异表明乙酰胆碱受体功能中特定脂质的调节作用。
The effect of specific lipids on the functional properties of the acetylcholine receptor were examined in reconstituted membranes prepared from purifiedTorpedo californicaacetylcholine receptor and various defined lipids. Cholesterol and negatively charged lipids greatly enhanced the ion influx response of the vesicles as measured by the effect of a receptor agonist on cation translocation across the vesicles. Part of the lipid-dependent effects could be attributed to alterations in the average size of the vesicles. All lipid mixtures used permitted complete incorporation of receptor and retention of ligand binding properties. Quantitative differences in ion flux properties suggest a modulating role for specific lipids in acetylcholine receptor function.