Transcriptional profiling of SNAI2 regulated genes in primary human keratinocytes.
Transcriptional profiling of SNAI2 regulated genes in primary human keratinocytes.
复制标题
DOI：
10.1016/j.gdata.2015.02.002
复制
发表时间：
2015-06-01
期刊：
影响因子：
--
通讯作者：
Sen, George L
中科院分区：
文献类型：
--
作者：
Mistry, Devendra S;Chen, Yifang;Wang, Ying;Sen, George L
已经发现上皮向间充质转化转录因子（EMT-TF）如SNAI 2在表皮干细胞和祖细胞中内源性表达，并且在分化时下调。祖细胞中SNAI 2的存在对于通过直接结合分化基因的启动子来抑制分化基因的表达是必需的。SNAI 2在分化后下调，这允许分化基因的表达。此外，SNAI 2的过表达可以阻断分化过程，表明SNAI 2的水平对表皮细胞命运决定至关重要。为了在全基因组水平上解决受改变SNAI 2水平影响的基因，我们对SNAI 2敲低和过表达表皮祖细胞进行了微阵列分析。在这里，我们提供了详细的方法和分析这些微阵列数据已存放在基因表达综合（GEO）：GSE 55269。
Epithelial to mesenchymal transition transcription factors (EMT-TFs) such as SNAI2 have been found to be expressed endogenously in epidermal stem and progenitor cells and downregulated upon differentiation. The presence of SNAI2 in progenitor cells is necessary to repress the expression of differentiation genes by binding directly to their promoters. SNAI2 is downregulated upon differentiation which allows expression of differentiation genes. Furthermore overexpression of SNAI2 can block the differentiation process suggesting that the levels of SNAI2 are crucial to epidermal cell fate decisions. To address on a genome wide level the genes that are impacted by changing the levels of SNAI2, we performed microarray analysis on SNAI2 knockdown and overexpressing epidermal progenitor cells. Here we provide detailed methods and analysis on these microarray data which has been deposited in Gene Expression Omnibus (GEO): GSE55269.