Fabrication of microporous inversion method as small material polyurethane by spray phase diameter vascular grafts
Fabrication of microporous inversion method as small material polyurethane by spray phase diameter vascular grafts
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DOI：
10.1002/jbm.a.30613
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发表时间：
2006-05-01
影响因子：
4.9
通讯作者：
Shorgashti, S
中科院分区：
文献类型：
--
作者：
Khorasani, MT;Shorgashti, S
采用喷雾相转化法制备了直径4 mm、壁厚0.3~0.4 mm的微孔聚氨酯血管假体。采用扫描电子显微镜(SEM)技术研究了喷枪与旋转芯棒之间的距离(SG)、旋转芯棒的转速(RRM)以及非溶剂种类对PU膜孔形态的影响。结果表明，随着SG与Rm之间距离的增加或Rm的减小，PU膜的孔隙率增大，拉伸强度降低，柔顺值增大。通过体积和血管直径的变化来衡量体外依从性。此外，当凝固剂(水)改为水/甲醇时，PU膜的孔隙率增加，多孔形态转变为丝状形态。在静止培养条件下，对贴壁依赖细胞，即L929成纤维细胞的贴壁进行了研究。用光学显微镜观察细胞黏附和细胞生长情况。结果表明，随着PU膜孔隙率的增加，泡孔生长也随之增加。(C)2005年威利期刊公司。
Microporous polyurethane vascular prostheses with a 4 mm diameter and 0.3-0.4 mm wall thickness were fabricated by a spray phase inversion technique. In this study, the effect of distance between spray guns (SG) and rotating mandrel (RM), the effect of rate of rotating mandrel (RRM), and the type of nonsolvent on pore morphology of PU films were evaluated using scanning electron microscopy (SEM) technique. It was observed that when the distance between SG and RM was increased or the rate of RM was decreased, the porosity of PU films increased and consequently the tensile strength decreased and compliance value increased. Compliance was measured in vitro by volume and vessel diameter changes. Furthermore, when the coagulant (water) was changed to the water/methanol, the porosity of PU film increased and porous morphology changed to filamentous morphology. Attachment of anchorage dependent cells, namely L929 fibroblast cells, were investigated in stationary culture conditions. The cells adhesion and cells growth were studied using optical photomicrographs. The results show that by increasing the porosity content of PU films would consequently increase the cell ingrowths. (c) 2005 Wiley Periodicals, Inc.