Proteome analysis of Lactobacillus helveticus H9 during growth in skim milk.
Proteome analysis of Lactobacillus helveticus H9 during growth in skim milk.
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DOI：
10.3168/jds.2014-8520
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发表时间：
2014-12
影响因子：
3.5
通讯作者：
Y. F. Chen;Y. F. Chen;Wenjing Zhao;R. N. Wu;R. N. Wu;Z. H. Sun;Z. H. Sun;Wenyi Zhang;Jicheng Wang;M. Bilige;H. P. Zhang;H. P. Zhang
中科院分区：
文献类型：
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作者：
Y. F. Chen;Y. F. Chen;Wenjing Zhao;R. N. Wu;R. N. Wu;Z. H. Sun;Z. H. Sun;Wenyi Zhang;Jicheng Wang;M. Bilige;H. P. Zhang;H. P. Zhang
瑞士乳杆菌H9是从西藏传统发酵牦牛乳(中国)中分离到的一株能在牛奶发酵过程中产生降压肽Val-Pro-Pro(VPP)和Ile-Pro-Pro(IPP)的菌株。为了了解Helveticus H9的蛋白质表达变化，采用双向凝胶电泳法(2-DE)分析了3个不同生长阶段的蛋白质组，即滞后期(pH 6.1)、对数期(pH 5.1)和稳定期(pH 4.5)。进一步分析发现，基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF/MS)鉴定出257个差异蛋白质点，鉴定出214个蛋白质点。差异表达蛋白的细胞功能比较复杂。有趣的是，仅在pH 5.1和4.5的图谱上观察到与蛋白质分解系统相关的蛋白质氨基肽酶N(PepN)、氨基肽酶E(Pepe)、内肽酶O2(PepO2)和寡肽运输系统渗透酶蛋白(OppC)，这与血管紧张素转换酶(ACE)抑制肽VPP和IPP在这两个生长阶段(对数期和稳定期)的存在是一致的。这些结果，结合先前对蛋白分解系统基因表达的研究，使我们得出结论，Opp运输系统、Pepe和PepO2可能与ACE抑制肽的产生有关。
Lactobacillus helveticus H9 was isolated from traditionally fermented yak milk in Tibet (China) with the ability to produce the antihypertensive peptides Val-Pro-Pro (VPP) and Ile-Pro-Pro (IPP) during milk fermentation. To understand the changes in the protein expression of L. helveticus H9, proteome analysis was performed at 3 different growth stages, lag phase (pH 6.1), log phase (pH 5.1), and stationary phase (pH 4.5) using 2-dimensional electrophoresis (2-DE). Further analysis showed that 257 differential protein spots were found and 214 protein spots were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The cellular functions of the differentially expressed proteins were complex. Interestingly, the proteolytic system-related proteins aminopeptidase N (PepN), aminopeptidase E (PepE), endopeptidase O2 (PepO2), and oligopeptide transport system permease protein (OppC) were observed only on the maps of pH 5.1 and pH 4.5, which was consistent with the presence of angiotensin I-converting enzyme (ACE)-inhibitory peptides VPP and IPP during these 2 growth stages (log phase and stationary phase). These results, combined with a previous study of gene expression of the proteolytic system, led us to conclude that the Opp transport system, pepE, and pepO2 are likely related to the production of ACE-inhibitory peptides.