Laser ablation studies of the role of the Drosophila oocyte nucleus in pattern formation.
Laser ablation studies of the role of the Drosophila oocyte nucleus in pattern formation.
复制标题
激光消融研究果蝇卵母细胞核在模式形成中的作用。
DOI：
10.1126/science.254.5029.290
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Spradling,AC
中科院分区：
文献类型：
--
作者：
Montell,DJ;Keshishian,H;Spradling,AC
体细胞和生殖细胞在卵子发生过程中相互作用，建立果蝇蛋壳和胚胎的模式轴。使用激光烧蚀测试了卵母细胞核在图案形成中的作用。第 6 至 9 阶段卵室的消融导致蛋壳部分或完全腹侧化，其表型与古尔克或鱼雷雌性所产的卵相似。经处理的卵母细胞后极的血管蛋白积累也被破坏。因此，卵母细胞核在卵母细胞内的背腹模式形成和卵母细胞中的极性血浆组装中需要晚至第9阶段。
Somatic and germline cells interact during oogenesis to establish the pattern axes of theDrosophilaeggshell and embryo. The role of the oocyte nucleus in pattern formation was tested with the use of laser ablation. Ablation in stage 6 to 9 egg chambers caused partial or complete ventralization of the eggshell, phenotypes similar to those of eggs produced bygurkenortorpedofemales. Accumulation ofvasaprotein at the posterior pole of treated oocytes was also disrupted. Thus the oocyte nucleus is required as late as stage 9 for dorsoventral patterning within the follicle cells and for polar plasm assembly in the oocyte.