Effects of light intensity on the production of VSLs from the marine diatom Ditylum brightwellii
Effects of light intensity on the production of VSLs from the marine diatom Ditylum brightwellii
复制标题
光强度对海洋硅藻 Ditylum Brightwellii 产生 VSL 的影响
DOI：
10.1007/s10874-021-09426-9
复制
发表时间：
2021
影响因子：
2
通讯作者：
Hashimoto Shinya
中科院分区：
文献类型：
--
作者：
Abe Minami;Okuda Yuki;Hashimoto Shinya
众所周知，极短寿命物质(VSLS)在对流层和平流层的臭氧消耗中起着重要作用。影响海洋浮游植物产生这些化合物的环境因素尚未得到很好的研究，海洋浮游植物被认为是这些化合物在公海中的来源。在这里，我们研究了光强对海洋硅藻Dit目Bright Walllii产生VSLS的影响。用吹扫捕集-气相色谱-质谱法测定了− -2S-− -1细胞在30、60、120微克分子光强下孵育的培养物中溴二氯甲烷、二溴氯甲烷、溴仿、氯仿和二溴甲烷的含量。通过测量叶绿素浓度和细胞密度来监测浮游植物的生长。叶绿素浓度(细胞密度)和VSLS的产生速率均随光照强度的增加而增加。CHBrCl2、CHBr2Cl3、CHBr3、CHCl3和CH2Br2的最大产生率在对数生长期和稳定期均达到最大值，但CH2Br2在− 2s− 1下孵育在30、60和120µmol光强下的最大产生率分别为0.06、0.46和1.84mol.− 2s− 1day−1day− 1.我们的结果表明，海洋硅藻是VSLS的重要来源之一，光强是估算公海VSLS排放的一个重要因素。
Very short-lived substances (VSLs) are known to play an important role in ozone depletion in the troposphere and stratosphere. Environmental factors that influence the production of these compounds by marine phytoplankton, which is known to be the source of these compounds in open oceans, have not yet been well studied. Here we examined the effects of light intensity on the production of VSLs by the marine diatomDitylum brightwellii. Bromodichloromethane (CHBrCl2), dibromochloromethane (CHBr2Cl), bromoform (CHBr3), chloroform (CHCl3), and dibromomethane (CH2Br2) in cultures incubated under full spectrum daylight intensities of 30, 60, and 120 µmol photons m− 2s− 1were measured using purge and trap gas chromatograph–mass spectrometry. Phytoplankton growth was monitored by measuring chlorophyll-aconcentration and cell density. Both the chlorophyll-aconcentration (the cell density) and the production rates of VSLs increased with increasing light intensity. The maximum production rates of CHBrCl2, CHBr2Cl, CHBr3, CHCl3, and CH2Br2were observed during the exponential or stationary phase, with the exception of CH2Br2incubated under 30 µmol photons m− 2s− 1. The chlorophylla-normalized (or cell-normalized) production rates of VSLs increased with increasing light intensity, e.g., the maximum of chlorophylla-normalized production rates of CHCl3under light intensities of 30, 60 and 120 µmol photons m− 2s− 1were 0.06, 0.46 and 1.84 µmol (g chlorophylla)−1day− 1, respectively. Our results suggest that marine diatoms are one of the significant sources of VSLs and that light intensity is a significant factor in estimating VSLs emissions from the open ocean.