Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts.
Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts.
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DOI：
10.1007/978-1-0716-2059-5_10
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发表时间：
2022
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在三维显微镜下研究时，健康和疾病中的血管和血液学变化更容易理解和解释。小鼠气管是一个简单，但功能强大，多功能的模型系统，在其中实现这一点。我们描述了实用的荧光和共聚焦显微镜的小鼠气管的wholemounts的免疫组织化学方法，以实现这一目的，其中整个脉管系统可以可视化从器官水平的细胞和亚细胞水平。血管和支气管具有高度定型的血管结构，在气管软骨之间的弧形中重复。小动脉、毛细血管和小静脉可以很容易地被识别为血管，而分支由初始分支和集合分支组成。即使是血管或神经系统中的微小异常也可以在三维空间中观察和评估。我们和其他人已经使用小鼠气管来检查原位血管生成和淋巴管生成、血管发育和退化、血管通畅性、转基因小鼠的差异以及病理变化，例如由炎症介质诱导的血管通透性增加。
Changes in blood vessels and lymphatics in health and disease are easier to understand and interpret when studied microscopically in three dimensions. The mouse trachea is a simple, yet powerful, and versatile model system in which to achieve this. We describe practical immunohistochemical methods for fluorescence and confocal microscopy of wholemounts of the mouse trachea to achieve this purpose in which the entire vasculature can be visualized from the organ level to the cellular and subcellular level. Blood vessels and lymphatics have highly stereotyped vascular architectures that repeat in arcades between the tracheal cartilages. Arterioles, capillaries, and venules can be easily identified for the blood vessels, while the lymphatics consist of initial lymphatics and collecting lymphatics. Even small abnormalities in either blood vessels or lymphatics can be noticed and evaluated in three dimensions. We and others have used the mouse trachea for examining in situ angiogenesis and lymphangiogenesis, vascular development and regression, vessel patency, differences in transgenic mice, and pathological changes, such as increased vascular permeability induced by inflammatory mediators.