Dynamic Assembly/Disassembly Processes of Photoresponsive DNA Origami Nanostructures Directly Visualized on a Lipid Membrane Surface
Dynamic Assembly/Disassembly Processes of Photoresponsive DNA Origami Nanostructures Directly Visualized on a Lipid Membrane Surface
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DOI：
10.1021/ja4109819
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发表时间：
2014-02-05
影响因子：
15
通讯作者：
Sugiyama, Hiroshi
中科院分区：
文献类型：
--
作者：
Suzuki, Yuki;Endo, Masayuki;Sugiyama, Hiroshi
在这里，我们报告了可以放置在脂质双层表面上的光响应 DNA 折纸纳米结构的组装/拆卸过程的直接可视化。该观察依赖于双层成分和引入六角形折纸结构的胆固醇部分之间的受控相互作用，六角形折纸结构的外边缘之一携带偶氮-ODN。双层放置的六角二聚体在紫外线照射下被分解成单体单元，并在可见光照射下再次可逆地组装。这些动态过程通过高速原子力显微镜直接监测。我们的方法的成功应用应该有助于研究各种 DNA 纳米结构的相互作用和功能行为。
Here, we report the direct visualization of the assembly/disassembly processes of photoresponsive DNA origami nanostructures which can be placed on a lipid bilayer surface. The observation relies on controlled interactions between the bilayer components and cholesterol moieties introduced to the hexagonal origami structures, one of whose outer edges carries Azo-ODNs. The bilayer-placed hexagonal dimer was disassembled into monomer units by UV irradiation, and reversibly assembled again during visible light irradiation. These dynamic processes were directly monitored with highspeed atomic force microscopy. The successful application of our approach should facilitate studies of interactive and functional behaviors of various DNA nanostructures.