Lipid-based carriers of microRNAs and intercellular communication.
Lipid-based carriers of microRNAs and intercellular communication.
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基于脂质的microRNA和细胞间通信。
DOI：
10.1097/mol.0b013e328350a425
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发表时间：
2012-04
影响因子：
4.4
通讯作者：
Remaley AT
中科院分区：
文献类型：
--
作者：
Vickers KC;Remaley AT
细胞外microRNAs(MiRNAs)在血浆中是唯一稳定的，循环中特定miRNAs的水平可能因疾病而异。细胞外miRNAs与脂基载体和无脂蛋白相关。MiRNAs可以通过基于脂质的载体在细胞间转移，并影响基因表达。本文综述了近年来有关miRNA在细胞间的转移及其在细胞间通讯中的潜在作用的研究进展。微泡、外切体、凋亡体、脂蛋白和大颗粒含有miRNAs。最近的研究表明，miRNAs可以在树突状细胞、肝癌细胞和脂肪细胞之间通过脂类载体进行转移。在脂质载体中，miRNAs也从T细胞转移到抗原提呈细胞，从干细胞转移到内皮细胞和成纤维细胞，从巨噬细胞转移到乳腺癌细胞，从上皮细胞转移到肝细胞。在脂质载体中，miRNAs的细胞输出受神经酰胺途径的调节，而脂质相关的miRNAs的传递是通过多种途径实现的，包括内吞摄取、膜融合和清道夫受体。细胞miRNAs输出到基于脂质的载体(囊泡和脂蛋白颗粒)中，并随着细胞间通讯而随着基因表达的变化而转移到受体细胞。
Extracellular microRNAs (miRNAs) are uniquely stable in plasma, and the levels of specific circulating miRNAs can differ with disease. Extracellular miRNAs are associated with lipid-based carriers and lipid-free proteins. miRNAs can be transferred from cell-to-cell by lipid-based carriers and affect gene expression. This review summarizes recent studies that demonstrate the transfer of miRNA between cells and their potential role in intercellular communication. Microvesicles, exosomes, apoptotic bodies, lipoproteins, and large microparticles contain miRNAs. Recent studies have demonstrated that miRNAs are transferred between dendritic cells, hepatocellular carcinoma cells, and adipocytes in lipid-based carriers. miRNAs are also transferred from T cells to antigen-presenting cells, from stem cells to endothelial cells and fibroblasts, from macrophages to breast cancer cells, and from epithelial cells to hepatocytes in lipid-based carriers. The cellular export of miRNAs in lipid-based carriers is regulated by the ceramide pathway, and the delivery of lipid-associated miRNAs to recipient cells is achieved by various routes, including endocytotic uptake, membrane-fusion, and scavenger receptors. Cellular miRNAs are exported in and to lipid-based carriers (vesicles and lipoprotein particles) and transferred to recipient cells with gene expression changes as intercellular communication.