ROLE OF MITOCHONDRIA IN GROWTH PLATE CALCIFICATION AS DEMONSTRATED IN A RACHITIC MODEL
ROLE OF MITOCHONDRIA IN GROWTH PLATE CALCIFICATION AS DEMONSTRATED IN A RACHITIC MODEL
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DOI：
10.2106/00004623-197860050-00007
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发表时间：
1978-01-01
影响因子：
5.3
通讯作者：
HUNT, RM
中科院分区：
文献类型：
--
作者：
BRIGHTON, CT;HUNT, RM
在大鼠磷酸盐-维生素D缺乏性佝偻病模型中，焦锑酸钾被用作在肋软骨生长板的超微结构水平上的Ca 2+的组织化学染色。在对照板中，有一个移动的Ca 2+从细胞内，主要是线粒体的位置在肥大细胞区的顶部到细胞外，主要是基质囊泡的位置在肥大细胞区的底部。在佝偻病板，线粒体和细胞膜整个底部的肥大区仍然严重负载Ca 2+。开始磷酸盐处理后，肥大区底部的线粒体和细胞膜迅速失去Ca 2+，基质开始钙化。因此，在正常的生长板中，基质钙化开始于板中线粒体失去Ca 2+的水平;在佝偻病中，基质不钙化，线粒体不失去Ca 2 +;在愈合的佝偻病中，钙化开始于肥大区底部的基质，因为该水平的线粒体失去Ca 2+。这些发现支持线粒体在基质钙化中起重要作用的假设。
In a phosphate-vitamin D deficiency rachitic model in rats, potassium pyroantimonate was employed as a histochemical stain for Ca2+ at the ultrastructural level in the costochondral growth plates. In the control plates, there was a shift of Ca2+ from an intracellular, mainly mitochondrial location in the top part of the zone of hypertrophic cells to an extracellular, mainly matrix-vesicle location in the bottom part of the zone of hypertrophic cells. In the rachitic plates, mitochondria and cell membranes throughout the bottom of the hypertrophic zone remained heavily loaded with Ca2+. After treatment with phosphate was started, the mitochondria and cell membranes at the bottom of the hypertrophic zone rapidly lost Ca2+ and matrix calcification began. Thus, in the normal growth plate, matrix calcification begins at the level in the plate where mitochondria lose Ca2+; in rickets, the matrix does not calcify and mitochondria do not lose Ca2+; and in healing rickets, calcification begins in the matrix at the bottom of the hypertrophic zone as the mitochondria at that level lose Ca2+. These findings support the hypothesis that mitochondria play an important role in matrix calcification.