Structure and activities of a novel heteroxylan from Cassia obtusifolia seeds and its sulfated derivative
Structure and activities of a novel heteroxylan from Cassia obtusifolia seeds and its sulfated derivative
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决明种子中新型异木聚糖及其硫酸化衍生物的结构和活性
DOI：
10.1016/j.carres.2014.04.016
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发表时间：
2014-07-01
影响因子：
3.1
通讯作者：
Ding, Kan
中科院分区：
文献类型：
--
作者：
Cong, Qifei;Shang, Mingsheng;Ding, Kan
从决明子种子的碱提物中分离得到COB 1B 1 S2，并通过阴离子交换和凝胶渗透色谱进行纯化。它含有阿拉伯糖、木糖和葡萄糖醛酸，摩尔比为5：81：14，表观分子量估计为70.4 kDa。通过使用化学和光谱方法阐明，COB 1B 1 S 2显示具有由1，4-连接的-D-Xylp组成的主链，其中几乎每五个1，4-连接的Xylp就有一个单单元末端α-D-GlcpA或α-L-Araf在O-2处被取代。COB 1B 1 S2在结构上与典型的葡萄糖醛酸葡聚糖不同，因为它在GlcA的O-4位没有甲基化。天然COB 1B 1 S2对人微血管内皮细胞（HMEC）的管形成以及对肝和结肠癌细胞的生长没有显示出显著的抑制。相反，作为COB 1B 1 S2的硫酸化衍生物制备的COB 1B 1 S2-Sul以剂量依赖性方式对HMEC的管形成和对Bel 7402肝癌细胞的生长表现出显著的抑制。这些结果表明，硫酸基团的引入显着提高了葡糖醛酸木聚糖的生物活性。(C)2014爱思唯尔有限公司版权所有。
COB1B1S2 was isolated from an alkaline extract of Cassia obtusifolia seeds, and purified by anion-exchange and gel permeation chromatography. It contains arabinose, xylose, and glucuronic acid, in the molar ratio of 5: 81: 14, with an apparent molecular weight estimated to be 70.4 kDa. Elucidated by using chemical and spectroscopic methods, COB1B1S2 was shown to have a backbone consisting of 1,4-linked-D-Xylp, with one single-unit terminal alpha-D-GlcpA or alpha-L-Araf substituted at O-2 for nearly every five 1,4-linked Xylp. COB1B1S2 is structurally different from typical glucuronoxylans by its absence of methylation at O-4 of GlcA. The native COB1B1S2 showed no significant inhibition on the tube formation of human microvascular endothelial cells (HMEC) and on the growth of liver and colon cancer cells. On the contrary, COB1B1S2-Sul, prepared as the sulfated derivative of COB1B1S2, exhibited a significant inhibition on tube formation of HMEC in a dose-dependent manner, and on the growth of Bel7402 liver cancer cells. These results indicated that the introduction of sulfate groups significantly enhanced the biological activity of glucuronoxylan. (C) 2014 Elsevier Ltd. All rights reserved.