Zymographic Characterization of Bacteriolytic Enzymes Produced by Oral Streptococci
Zymographic Characterization of Bacteriolytic Enzymes Produced by Oral Streptococci
复制标题
口腔链球菌产生的溶菌酶的酶谱表征
DOI：
10.1111/j.1348-0421.2004.tb03537.x
复制
发表时间：
2004
影响因子：
2.6
通讯作者：
M. Sugai
中科院分区：
文献类型：
--
作者：
Goh Yoshimura;H. Komatsuzawa;Junko Kajimura;T. Fujiwara;M. Ohara;K. Kozai;M. Sugai
对口腔链球菌、变形链球菌、远缘链球菌、血链球菌、米氏链球菌和唾液链球菌4%十二烷基硫酸钠提取液进行酶谱分析。我们对每个物种中的五个菌株进行了调查，发现除了唾液葡萄球菌外，同一物种的菌株之间的图谱非常相似(图谱分为两种类型)。另一方面，不同物种的情况也有很大的不同。以变性链球菌和远缘链霉菌为底物，发现两种主要的溶菌酶，其相对分子质量分别为80和100 kDa。这些溶菌活性在含有金黄色葡萄球菌或唾液葡萄球菌的凝胶中不太明显，在含有血链球菌的凝胶中也检测不到。远缘链霉菌提取液以远缘链霉菌细胞为底物，只显示一条较强的溶菌带(78 KDa)。血链球菌提取物对任何链球菌细胞均无溶菌带。以各自的菌株为底物，米氏链霉菌和唾液链霉菌的提取物均表现出较弱的活性。
Zymographic analysis was performed to know the bacteriolytic enzyme profiles of 4% SDS extracts of oral streptococci, Streptococcus mutans, S. sobrinus, S. sanguis, S. mitis and S. salivarius. We investigated the five strains in each species and found that the profile was very similar among strains of the same species except for S. salivarius (the profile was classified into two types). On the other hand, the profile was considerably different among species. Two major bacteriolytic enzymes of S. mutans showing molecular mass of 80 and 100 kDa were found using SDS‐boiled S. mutans or S. sobrinus cells as substrate. These bacteriolytic activities were less apparent in the gel containing S. mitis or S. salivarius, and also not detectable in the gel containing S. sanguis. S. sobrinus extract showed only one bacteriolytic band (78 kDa) as strong activity using S. sobrinus cells as substrate. S. sanguis extract showed no bacteriolytic bands using any streptococcal cells. Extracts of either S. mitis or S. salivarius showed weak activity by using respective strains as substrate.