Determination of the orientation of the magnetic axes of the cyano-MetMb complexes of point mutants of myoglobin by solution 1H NMR : influence of His E7 → Gly and Arg CD3 → Gly substitutions
Determination of the orientation of the magnetic axes of the cyano-MetMb complexes of point mutants of myoglobin by solution 1H NMR : influence of His E7 → Gly and Arg CD3 → Gly substitutions
复制标题
通过溶液 1H NMR 测定肌红蛋白点突变体的氰基-MetMb 复合物的磁轴方向：His E7 → Gly 和 Arg CD3 → Gly 取代的影响
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
S. Sligar
中科院分区：
文献类型：
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作者：
K. Rajarathnam;G. N. Mar;M. Chiu;S. Sligar
本文描述了一种获取含铁抹香鲸肌红蛋白（metMbCN）远端突变体低自旋氰配合物对角线顺磁化率张量轴的方法。它依赖于使用野生型（WT）蛋白的晶体坐标，对于未受点突变干扰的那部分分子，加上实验偶极位移，来寻找正确地将晶体坐标转换为磁轴的欧拉旋转
A method is described for obtaining the axes of the diagonal paramagnetic susceptibility tensor for the low-spin cyanide complexes of distal point mutants of ferric sperm whale myoglobin (metMbCN). It relies on using the crystal coordinates of the wild-type (WT) protein for that portion of the molecule unperturbed by the point mutation, together with the experimental dipolar shifts, to search for the Euler rotation that correctly converts the crystal coordinates to the magnetic axes