Airborne Prokaryotic, Fungal and Eukaryotic Communities of an Urban Environment in the UK
Airborne Prokaryotic, Fungal and Eukaryotic Communities of an Urban Environment in the UK
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DOI：
10.3390/atmos13081212
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发表时间：
2022-08
期刊：
影响因子：
2.9
通讯作者：
Hokyung Song;N. Marsden;J. Lloyd;C. Robinson;C. Boothman;I. Crawford;M. Gallagher;H. Coe;Grant Allen;M. Flynn
中科院分区：
文献类型：
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作者：
Hokyung Song;N. Marsden;J. Lloyd;C. Robinson;C. Boothman;I. Crawford;M. Gallagher;H. Coe;Grant Allen;M. Flynn
生物气溶胶通常含有影响公众健康的人类病原体和过敏原。然而，与非生物气溶胶相比，对生物气溶胶的关注相对较少。在这项研究中，我们的目的是确定生物气溶胶组成在曼彻斯特，英国通过应用高通量测序方法，并找到潜在的来源。于2019年11月及2020年2月在曼彻斯特空气质量超级站点的Firs环境研究站收集样本。提取总DNA并针对原核生物的16S rRNA基因、真菌DNA的ITS区和真核生物的18S rRNA基因进行测序。我们发现，与2019年11月的样本相比，2020年2月的样本中与海洋环境相关的细菌和古菌相对更丰富，这与基于风反向轨迹分析的西北海洋起源一致。相比之下，属于甲基杆菌属的OTU（包括许多对重金属具有抗性的物种）在2019年11月金属浓度较高时相对更丰富。在2020年2月的样本中，全年结果的真菌类群相对更丰富，而在2019年11月的样本中，秋季结果物种的相对丰度通常更高。根据18S rRNA基因测序，2020年2月的样品中陆生植物和藻类的相对丰度较高。二零二零年二月的样本中，其中一个属于针叶红豆杉属的OTU较丰富，与英国红豆杉通常的花粉季节（一月中旬至四月下旬）一致。这项研究的结果表明，生物气溶胶剖面追踪大气颗粒物的来源的潜在应用。
Bioaerosols often contain human pathogens and allergens affecting public health. However, relatively little attention has been given to bioaerosols compared with non-biological aerosols. In this study, we aimed to identify bioaerosol compositions in Manchester, UK by applying high throughput sequencing methods and to find potential sources. Samples were collected at Manchester Air Quality Super Site at the Firs Environmental Research Station in November 2019 and in February 2020. Total DNA has been extracted and sequenced targeting the 16S rRNA gene of prokaryotes, ITS region of fungal DNA and 18S rRNA gene of eukaryotes. We found marine environment-associated bacteria and archaea were relatively more abundant in the February 2020 samples compared with the November 2019 samples, consistent with the North West marine origin based on wind back-trajectory analysis. In contrast, an OTU belonging to Methylobacterium, which includes many species resistant to heavy metals, was relatively more abundant in November 2019 when there were higher metal concentrations. Fungal taxa that fruit all year were relatively more abundant in the February 2020 samples while autumn fruiting species generally had higher relative abundance in the November 2019 samples. There were higher relative abundances of land plants and algae in the February 2020 samples based on 18S rRNA gene sequencing. One of the OTUs belonging to the coniferous yew genus Taxus was more abundant in the February 2020 samples agreeing with the usual pollen season of yews in the UK which is from mid-January until late April. The result from this study suggests a potential application of bioaerosol profiling for tracing the source of atmospheric particles.