THE CORNEA AND CORNEAL ENDOTHELIUM IN THE AGED RABBIT
THE CORNEA AND CORNEAL ENDOTHELIUM IN THE AGED RABBIT
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DOI：
10.1097/00006324-199412000-00013
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发表时间：
1994-12-01
影响因子：
1.4
通讯作者：
DOUGHTY, MJ
中科院分区：
文献类型：
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作者：
DOUGHTY, MJ
兔常用于角膜研究，但关于年龄影响的数据很少，特别是对角膜内皮形态的影响。 进行了一项探索性研究，以评估角膜尺寸和厚度以及内皮细胞密度（ECD）随年龄的变化。 将雌性新西兰白色（NZW）家兔单独圈养在饲料、温度、湿度和光照受控条件下长达3年。 体重从6周时的1 kg增加至3年时的6 kg。 水平角膜直径从6周时的11.34 mm增加到3年时的16.75 mm，而角膜厚度在同一时期从300 μ m增加到465 μ m。 ECD从约4100个细胞/mm 2下降到6个月时低于3000个细胞/mm 2，2年时低于2000个细胞/mm 2。 扫描电子显微镜（SEM）评价显示，根据内皮镶嵌的区域差异评估，轻度内皮多形畸形可能在9至12个月时检测到。 对于在老化角膜研究中使用的兔子，最小年龄为9个月（即，重量约为4.25 kg）。
The rabbit is commonly used in corneal research yet few data are available concerning the impact of age, especially on the morphology of the corneal endothelium. An exploratory study was undertaken to assess corneal dimensions and thickness and endothelial cell density (ECD) as a function of age. Female New Zealand White (NZW) rabbits were individually housed in diet-, temperature-, humidity-, and light-controlled conditions for up to 3 years. Body weight increased from 1 kg at 6 weeks to 6 kg at 3 years. Horizontal corneal diameter increased from 11.34 mm at 6 weeks to 16.75 mm at 3 years, whereas corneal thickness increased from 300 to 465 mum over the same time period. ECD decreased from approximately 4100 cells/mm2 to less than 3000 cells/mm2 by 6 months and to less than 2000 cells/mm2 by 2 years. Scanning electron microscopy (SEM) evaluations revealed that mild endothelial polymegethism, as assessed by regional variance in the endothelial mosaic, was likely to be detectable by 9 to 12 months. For the use of the rabbit in studies of the aged cornea, a minimum age of 9 months (i.e., approximately 4.25 kg in weight) is therefore recommended.