Short- and long-term exposure to alternating magnetic field (50 Hz, 0.5 mT) affects rat pituitary ACTH cells: Stereological study
Short- and long-term exposure to alternating magnetic field (50 Hz, 0.5 mT) affects rat pituitary ACTH cells: Stereological study
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DOI：
10.1002/tox.22059
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发表时间：
2016-04-01
影响因子：
4.5
通讯作者：
Petkovic, Branka
中科院分区：
文献类型：
--
作者：
Balind, Snezana Raus;Manojlovic-Stojanoski, Milica;Petkovic, Branka
本研究的目的是确定极低频磁场（ELF-MF，50 Hz，0.5 mT）是否影响成年动物垂体促肾上腺皮质激素（ACTH）细胞。我们进行了两个系列的实验：（1）3个月大的大鼠短期暴露于ELF-MF 1和7天，和（2）大鼠长期暴露于ELF-MF从他们的概念到3个月大。对免疫标记的垂体ACTH细胞进行体视学研究。测量ACTH细胞总数、体积、细胞核体积及垂体体积。ELF-MF暴露1天显着减少ACTH细胞的总数和体积，其核的体积，以及垂体体积。ELF-MF暴露7天仅显着减少了ACTH细胞的体积。终身暴露于ELF-MF诱导ACTH细胞和垂体体积的体积减少。我们可以得出结论，应用ELF-MF对垂体ACTH细胞的形态计量学参数有很强的影响，可以被认为是一个应激因素。(c)2014 Wiley Periodicals，Inc.环境毒理学31：461-468，2016年。
The aim of the present study was to determine does extremely low frequency magnetic field (ELF-MF, 50 Hz, 0.5 mT) affect pituitary adrenocorticotroph (ACTH) cells in adult animals. We performed two series of experiments: (1) short-term exposure of 3-month-old rats to ELF-MF for 1 and 7 days, and (2) long-term exposure of rats to ELF-MF from their conception to 3 months of age. Stereological study was performed on immunolabeled pituitary ACTH cells. The total number and volume of ACTH cells, the volume of their nuclei and pituitary volume were measured. ELF-MF exposure for 1 day significantly decreased total number and volume of ACTH cells, the volume of their nuclei, as well as pituitary volume. ELF-MF exposure for 7 days significantly reduced only the volume of ACTH cells. Life-long exposure to ELF-MF induced decrease in the volume of ACTH cells and pituitary volume. We can conclude that the applied ELF-MF has a strong influence on morphometrical parameters of the pituitary ACTH cells and could be considered as a stressogenic factor. (c) 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 461-468, 2016.