Development of resistance during antimicrobial therapy caused by insertion sequence interruption of porin genes
Development of resistance during antimicrobial therapy caused by insertion sequence interruption of porin genes
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DOI：
10.1128/aac.43.4.937
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发表时间：
1999-04-01
影响因子：
4.9
通讯作者：
Albertí, S
中科院分区：
文献类型：
--
作者：
Hernández-Allés, S;Benedí, VJ;Albertí, S
我们已经证明，通过使用体外方法，插入序列（IS）的OmpK36孔蛋白基因的中断是一种常见的突变类型，导致孔蛋白表达的丢失和肺炎克雷伯氏菌对头孢西丁的耐药性增加。这一机制在体内也起作用：在13株孔蛋白缺陷型头孢西丁耐药临床分离的克雷伯氏菌中，pneumoniae，4例ompK36基因中存在IS。
We have demonstrated by using an in vitro approach that interruption of the OmpK36 porin gene by insertion sequences (ISs) is a common type of mutation that causes toss of porin expression and increased resistance to cefoxitin in Klebsiella pneumoniae. This mechanism also operates in vivo: of 13 porin-deficient cefoxitin-resistant clinical isolates of K. pneumoniae, 4 presented ISs in their ompK36 gene.