PHOTOINACTIVATION OF VIRUSES IN HUMAN FRESH PLASMA BY PHENOTHIAZINE DYES IN COMBINATION WITH VISIBLE-LIGHT
PHOTOINACTIVATION OF VIRUSES IN HUMAN FRESH PLASMA BY PHENOTHIAZINE DYES IN COMBINATION WITH VISIBLE-LIGHT
复制标题
DOI：
10.1111/j.1423-0410.1991.tb00907.x
复制
发表时间：
1991-01-01
期刊：
影响因子：
2.7
通讯作者：
SCHMITT, H
中科院分区：
文献类型：
--
作者：
LAMBRECHT, B;MOHR, H;SCHMITT, H
我们开发了一种光动力方法来灭活人类新鲜血浆中的病毒。 在低剂量吩噻嗪染料（如亚甲蓝或甲苯胺蓝）存在下，用可见光照射单个血浆袋。 通过这种治疗，包括艾滋病病原体HIV-1在内的不同包膜病毒的感染性可以从血浆中完全去除。 然而，无包膜病毒被证明更稳定。 凝血因子和其他血浆蛋白的活性仅略有下降。 没有迹象表明该过程导致血浆蛋白发生重要的结构修饰。 这些染料在浓度远低于它们在治疗高铁血红蛋白血症时用作解毒剂的浓度时具有光动力活性。
We developed a photodynamic method to inactivate viruses in human fresh plasma. Single plasma bags were illuminated with visible light in the presence of low doses of phenothiazine dyes like methylene blue or toluidine blue. By this treatment the infectivity of different enveloped viruses including the causative agent of AIDS, HIV-1, was completely removable from the plasma. Non enveloped viruses, however, proved to be more stable. The activities of clotting factors and other plasma proteins were only slightly decreased. There was no indication that the procedure led to important structural modifications of plasma proteins. The dyes are photodynamically active at concentrations much lower than those at which they are therapeutically used as antidots in the treatment of methemoglobinemia.