Differential Cortical Laminar Structure Revealed by NeuN Immunostaining and Myeloarchitecture Between Sulcal and Gyral Regions Independent of Sexual Dimorphisms in the Ferret Cerebrum
Differential Cortical Laminar Structure Revealed by NeuN Immunostaining and Myeloarchitecture Between Sulcal and Gyral Regions Independent of Sexual Dimorphisms in the Ferret Cerebrum
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DOI：
10.1002/ar.23369
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发表时间：
2016-08-01
影响因子：
2
通讯作者：
Sawada, Kazuhiko
中科院分区：
文献类型：
--
作者：
Horiuchi-Hirose, Miwa;Sawada, Kazuhiko
本研究的目的是定量阐明雪貂大脑皮层脑沟和脑回区域的板层结构和髓构筑的差异。取出生后90d的雄性和雌性雪貂，在冠状平面上制作大脑组织切片，进行抗NeuN或抗髓鞘碱性蛋白(MBP)免疫组织化学染色。在具有代表性的5个脑沟和7个脑回区域的新皮质的整个深度或三层，即I层、外层(II-III层)和内层(IV-VI层)的厚度被估计。与整个皮质深度一样，沟底部的外层和内层明显比回牙冠薄，而I层的厚度大约是沟底部的两倍。然而，在检查的五个脑沟区域或七个脑回区域中，整个皮质深度和每一皮质层的厚度没有统计学差异。经MBP免疫组织化学染色，髓鞘纤维呈切线状穿过回牙冠I层浅层区域。这些纤维在额叶和颞叶回中密度相对较高，在顶叶和枕叶回中相对稀疏，但各回的密度无性别差异。这些结果表明，雌雄不同的雪貂大脑皮质的沟区和回区之间存在着不同的层状结构和髓构筑。I层切向纤维的髓鞘形成在初级回中不同，在系统发育的高级皮质回中较弱。Anat Rec，299：1003-1011,2016。(C)2016威利期刊公司。
The purpose of this study was to quantitatively clarify differences in laminar structure and myeloarchitecture of sulcal and gyral regions of the cerebral cortex of ferrets. Histological sections of cerebrum from male and female ferrets at postnatal day 90 were made at the coronal plane, and were immunostained with anti-NeuN or anti-myelin basic protein (MBP). Thickness was estimated in the entire depth or three strata, that is, layer I, outer (layers II-III) and inner (layers IV-VI) strata of the neocortex in representative five sulcal and seven gyral regions. As with the entire cortical depth, outer and inner strata were significantly thinner in the sulcal bottoms than in the gyral crowns, whereas layer I had about twofold greater thickness in the sulcal bottoms. However, thicknesses of the entire cortical depth and each cortical stratum were not statistically different among five sulcal regions or seven gyral regions examined. By MBP immunostaining, myelin fibers ran tangentially through the superficial regions of layer I in gyral crowns. Those fibers were relatively denser in gyri of frontal and temporal regions, and relatively sparse in gyri of parietal and occipital regions, although their density in any gyri was not different between sexes. These results show a differential laminar structure and myeloarchitecture between the sulcal and gyral regions of the ferret cerebral cortex present in both sexes. Myelination of layer I tangential fibers varied among primary gyri and was weaker in phylogenetically higher-order cortical gyri. Anat Rec, 299:1003-1011, 2016. (C) 2016 Wiley Periodicals, Inc.