Goosecoid is not an essential component of the mouse gastrula organizer but is required for craniofacial and rib development.
Goosecoid is not an essential component of the mouse gastrula organizer but is required for craniofacial and rib development.
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发表时间：
1995-09
期刊：
影响因子：
4.6
通讯作者：
Jaime A. Rivera-Pérez;Moisés Mallo;M. Gendron-Maguire;Thomas Gridley;Richard R. Behringer
中科院分区：
文献类型：
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作者：
Jaime A. Rivera-Pérez;Moisés Mallo;M. Gendron-Maguire;Thomas Gridley;Richard R. Behringer
鹅样蛋白（Goosecoid, gsc）是一种进化保守的同源盒基因，表达于多种脊椎动物胚胎的原肠胚组织区，包括斑马鱼、爪蟾、鸡和小鼠。为了了解gsc在小鼠胚胎发生中的作用，我们在胚胎干细胞中通过基因靶向产生了gsc缺失的小鼠。令人惊讶的是，gsc缺失的胚胎原肠胚形成并形成了初级体轴；gsc缺失的小鼠出生时存活，但出生后不久就死亡，伴有大量颅面缺陷。此外，检测到肋骨融合和胸骨异常，这取决于遗传背景。移植实验表明卵巢不提供gsc功能来修复原肠胚发育缺陷。这些结果表明，gsc在小鼠的组织者活动中不是必需的，但在胚胎发生后期颅面和胸腔发育中是必需的。
Goosecoid (gsc) is an evolutionarily conserved homeobox gene expressed in the gastrula organizer region of a variety of vertebrate embryos, including zebrafish, Xenopus, chicken and mouse. To understand the role of gsc during mouse embryogenesis, we generated gsc-null mice by gene targeting in embryonic stem cells. Surprisingly, gsc-null embryos gastrulated and formed the primary body axes; gsc-null mice were born alive but died soon after birth with numerous craniofacial defects. In addition, rib fusions and sternum abnormalities were detected that varied depending upon the genetic background. Transplantation experiments suggest that the ovary does not provide gsc function to rescue gastrulation defects. These results demonstrate that gsc is not essential for organizer activity in the mouse but is required later during embryogenesis for craniofacial and rib cage development.