Comparative analysis of human papillomavirus detection by polymerase chain reaction and Virapap/Viratype kits.
Comparative analysis of human papillomavirus detection by polymerase chain reaction and Virapap/Viratype kits.
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聚合酶链反应和 Virapap/Viratype 试剂盒检测人乳头瘤病毒的比较分析。
DOI：
10.1093/ajcp/94.5.554
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发表时间：
1990
影响因子：
3.5
通讯作者：
Kulander,BG
中科院分区：
文献类型：
--
作者：
Burmer,GC;Parker,JD;Bates,J;East,K;Kulander,BG
通过聚合酶链反应（PCR）和Southern杂交，分析了270例由地区医生转诊的妇女宫颈标本中人乳头瘤病毒（HPV）6、11、16和18型的存在。通过商业滤膜杂交技术（Virapap®和Viratype®试剂盒，Life Technologies，Bethesda Research Labs，盖瑟斯堡，MD）同时分析1 S4例患者的样本。Southern杂交结合PCR的灵敏度明显高于Virapap和Viratype法。PCR检测结果为异型增生阳性的妇女中有67%检出HPV，Virapap法为47%。在每个诊断类别中，HPV 16/18型比6/11型更常见。在12%的HPV阳性患者中检测到一种以上的HPV类型。通过PCR测量，细胞学阴性或不确定患者的HPV患病率分别为22%和40%;相比之下，通过Virapap方法，这些值分别为7%和10%。这些结果表明，PCR与Southern杂交相结合提供了比使用杂交而不扩增HPV DNA的方法更高水平的灵敏度，并且还表明HPV的流行率在细胞学阳性涂片中最高。
Cervical samples from 270 women referred by area physicians were analyzed for the presence of human papillomavirus (HPV) types 6,11,16, and 18 by the polymerase chain reaction (PCR) followed by Southern hybridization. Samples from 1S4 patients were concurrently analyzed by a commercial filter hybridization technique (Virapap® and Viratype® Kits, Life Technologies, Bethesda Research Labs, Gaithersburg, MD). The sensitivity of the Southern blot procedure combined with PCR was significantly higher than that of the Virapap and Viratype methods. HPV was detected in 67% of women who had positive results for dysplasia by PCR and in 47% by the Virapap method. HPV types 16/18 were found more commonly than types 6/11 in every diagnostic category. More than one HPV type was detected in 12% of HPV-positive patients. The prevalence of HPV in cytologically negative or indefinite patients as measured by PCR was 22% and 40%; in contrast, by the Virapap method, these values were 7% and 10%. These results demonstrate that PCR combined with Southern hybridization provides a higher level of sensitivity than methods that use hybridization without amplification of HPV DNA and also show that the prevalence of HPV is highest in cytologically positive smears.