Comparison of the Kato-Katz and FLOTAC techniques for the diagnosis of soil-transmitted helminth infections
Comparison of the Kato-Katz and FLOTAC techniques for the diagnosis of soil-transmitted helminth infections
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DOI：
10.1016/j.parint.2011.06.020
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发表时间：
2011-12-01
影响因子：
1.9
通讯作者：
Erko, Berhanu
中科院分区：
文献类型：
--
作者：
Habtamu, Kassahun;Degarege, Abraham;Erko, Berhanu
决定个人或社区的治疗和化疗为基础的控制程序的评价取决于寄生虫学诊断技术。本研究的目的是比较单次Kato-Katz厚涂片和单次FLOTAC测定土源性蠕虫感染的患病率和强度的准确性。从埃塞俄比亚的学龄儿童中共收集了271份粪便标本，并使用Kato-Katz方法（每张载玻片41.7 mg粪便）和FLOTAC技术进行显微镜检查。Kato-Katz和FLOTAC方法的组合结果用作诊断“金”标准，用于分析中的参考。两种方法之间的一致性显示蛔虫、鞭虫和钩虫的kappa值分别为0.74、0.73和0.28。对于三种土传蠕虫中的每一种，单个FLOTAC显示出比单个Kato-Katz显著更多的感染（p < 0.01）。单个Kato-Katz诊断T. trichiura、毛尾拟步行虫A.蛔虫和钩虫感染率分别为76.6%、67.8%和19.6%，而FLOTAC对3种土源性蠕虫的敏感性均为100%。单头Kato-Katz产卵量（729.1、145.2和60.7个卵/克粪便）显著高于单头Kato-Katz产卵量（729.1、145.2和60.7个卵/克粪便）。lumbricoides，T.鞭虫和钩虫）与单一FLOTAC（分别为142.5、54.5和14.6 EPG）相比（p < 0.05）。我们的研究证实，一个单一的FLOTAC是更敏感的比一个单一的加藤Katz土壤传播的蠕虫感染的诊断，但结果在较低的FEC。在推荐FLOTAC用于大规模社区诊断之前，在不同的流行病学背景下，在不同的感染强度水平下，仍需要进一步的标准化和验证。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Decisions on individual or community treatment and evaluation of chemotherapy based control programs depend on parasitological diagnostic techniques. The aim of this study was to compare the accuracy of a single Kato-Katz thick smear and a single FLOTAC for the determination of the prevalence and intensity of soil-transmitted helminth infections. A total of 271 faecal specimens were collected from schoolchildren in Ethiopia, and microscopically examined using the Kato-Katz method (41.7 mg stool per slide) and the FLOTAC technique. The combined results from the Kato-Katz and FLOTAC methods were used as diagnostic 'gold' standard for reference in the analysis. Agreement between the two methods showed kappa values of 0.74, 0.73 and 0.28 for Ascaris lumbricoides, Trichuris trichiura and hookworm, respectively. A single FLOTAC revealed significantly more infections than a single Kato-Katz for each of the three soil-transmitted helminths ( p < 0.01). The sensitivities of a single Kato-Katz for diagnosis of T. trichiura, A. lumbricoides and hookworm infections were 76.6%, 67.8% and 19.6%, respectively, while the sensitivity of FLOTAC was 100% for all the three soil-transmitted helminth species. A single Kato-Katz yielded considerably higher mean faecal egg counts (FECs) (729.1, 145.2 and 60.7 eggs per gram of stool (EPG) for A. lumbricoides, T. trichiura and hookworm, respectively) compared with a single FLOTAC (142.5, 54.5 and 14.6 EPG, respectively) ( p < 0.05). Our study confirms that a single FLOTAC is more sensitive than a single Kato-Katz for the diagnosis of soil-transmitted helminth infections, but results in lower FECs. Further standardization and validation are still required in different epidemiological settings with varying levels of intensity of infections before recommending FLOTAC for large-scale community diagnosis. (C) 2011 Elsevier Ireland Ltd. All rights reserved.