Novel developmental boundary in the cerebellum revealed by zebrin expression in the lurcher (Lc/+) mutant mouse.
Novel developmental boundary in the cerebellum revealed by zebrin expression in the lurcher (Lc/+) mutant mouse.
复制标题
通过 lucher (Lc/ ) 突变小鼠中斑马蛋白的表达揭示了小脑的新发育边界。
DOI：
10.1002/cne.903230111
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hawkes,R
中科院分区：
文献类型：
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作者：
Tano,D;Napieralski,JA;Eisenman,LM;Messer,A;Plummer,J;Hawkes,R
小脑皮质包含至少两类浦肯野细胞，它们被组织成交替排列的旁索带。这种区室化最清楚的证明是一个多肽抗原家族的表达模式，即由浦肯野细胞亚群选择性表达的zebrin。此外，顺行追踪实验表明，zeolite车厢密切相关的传入和传出的投影地图。进一步细分的长旁带成更小的模块可能会发生通过几种不同的机制，包括固有的小脑小叶和传入终末场的选择性分布。然而，虽然纵向细分是直截了当地显示，内外侧边界更微妙。在这份报告中，我们描述了一种新的中外侧和前后区室边界的小鼠，贯穿小叶VIII，这是揭示了后果的thelurcher（Lc/+）等位基因的zerobic表达。在正常小鼠中，zekin的区室化在几个不连续的阶段中发展：直到出生后第5天（PD 5），zekin没有表达;从PD 5-PD 7 zekin仅在后叶蚓部发现，免疫反应性浦肯野细胞在小叶X、IX和VIII中，但在其他地方没有;从PD 7-PD 12开始，蚓部的大多数浦肯野细胞变为zerotin +;从PD 12-PD 15开始，免疫反应性也出现在半球中，因此现在几乎所有浦肯野细胞都是zerotin +最后，从PD 15-PD 25开始，在成年时为Zeolites的浦肯野细胞中Zeolites逐渐受到抑制，直到显示出成熟的旁室模式。在Lc/+突变体中，正常的发育进程在PD 7左右中断。因此，当免疫反应性浦肯野细胞仅限于后叶蚓部时，zeolite表达的模式在该阶段变得冻结。表达区和非表达区之间的可重复边界在小叶VIII的背侧表面的mediolatively。除了zebrin表达本身之外，这种转变没有明显的结构相关性。这个内外边界确定了小脑后叶蚓部的发育单位，并进一步证明小脑是一个高度异质性的结构。© 1992 Wiley利斯公司
The cerebellar cortex contains at least two classes of Purkinje cells, which are organized into alternating arrays of parasagittal bands. The clearest demonstration of this compartmentation is the pattern of expression of a family of polypeptide antigens, the zebrins, which are expressed selectively by Purkinje cell subsets. Furthermore, anterograde tracing experiments show that the zebrin compartments are closely correlated with both afferent and efferent projection maps. The further subdivision of long parasagittal bands into smaller modules may occur through several different mechanisms, including the intrinsic cerebellar lobulation and the selective distribution of afferent terminal fields. However, while the longitudinal subdivisions are straightforwardly shown, the mediolateral boundaries are more subtle. In this report we describe a novel mediolateral and anteroposterior compartmentation boundary in mice, running across lobule VIII, that is revealed by the consequences of thelurcher(Lc/+) allele for zebrin expression. In normal mice zebrin compartmentation develops in several discrete stages: until postnatal day 5 (PD5) there is no zebrin expression; from PD5–PD7 zebrin is found only in the posterior lobe vermis, with immunoreactive Purkinje cells in lobules X, IX, and VIII but not elsewhere; from PD7–PD12 most Purkinje cells in the vermis become zebrin+ from PD12–PD15 immunoreactivity also appears in the hemispheres so that almost all Purkinje cells now are zebrin+ and finally, from PD15–PD25 zebrin is gradually suppressed in those Purkinje cells that are zebrin‐ in the adult until the mature pattern of parasagittal compartments is revealed. In theLc/+ mutant the normal developmental progression is interrupted at around PD7. As a result, the pattern of zebrin expression becomes frozen at that stage when immunoreactive Purkinje cells are confined exclusively to the posterior lobe vermis. A reproducible boundary between expressing and nonexpressing zones runs mediolaterally across the dorsal surface of lobule VIII. Apart from zebrin expression itself, there are no obvious structural correlates of this transition. This mediolateral boundary identifies a developmental unit in the posterior lobe vermis of the cerebellum, and provides further evidence that the cerebellum is a highly heterogeneous structure. © 1992 Wiley‐Liss, Inc.