Twenty-Four Hour Human Urine and Serum Profiles of Bisphenol A during High-Dietary Exposure
Twenty-Four Hour Human Urine and Serum Profiles of Bisphenol A during High-Dietary Exposure
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DOI：
10.1093/toxsci/kfr160
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发表时间：
2011-09-01
影响因子：
3.8
通讯作者：
Graham, Morgan K.
中科院分区：
文献类型：
--
作者：
Teeguarden, Justin G.;Calafat, Antonia M.;Graham, Morgan K.
由于双酚A与类固醇激素受体结合，双酚A(BPA，未结合的生物活性单体)在体内存在足够数量时被假设为雌激素样物质，这引发了人们对广泛接触BPA可能影响人类健康的担忧。为了更好地了解成人体内暴露于双酚A的情况，以及双酚A的血、尿药代动力学之间的关系，进行了一项临床暴露研究。血液和尿液样本在24小时内从20名成年志愿者身上采集，他们100%摄入了三种指定膳食中的一种，包括标准杂货店早餐、午餐和晚餐的食物。志愿者的BPA平均摄入量为0.27微克/千克体重(范围为0.03-0.86)，比美国成年人总暴露量的第95个百分位数高出21%。(TOT)BPA的血清时间进程仅在暴露于比美国总暴露的第95个百分位数高1.3-3.9倍的个人中观察到。(TOT)BPA餐后尿药浓度Tmax为2.75h(范围0.75~5.75h)，滞后于血药浓度Tmax约1h，每单位BPA暴露曲线下面积为21.5~79.0 nm中心点h中心点kg/mgBPA。血清(TOT)BPA浓度从小于或等于检测下限(LOD，1.3 nM)到5.7 nM，平均低于尿液浓度的42倍。在这些高饮食暴露期间，在收集的320个样本中，83%的血清中未检测到(TOT)双酚A浓度，所有样本的双酚A浓度均被确定为小于或等于LOD。
By virtue of its binding to steroid hormone receptors, bisphenol A (BPA, the unconjugated bioactive monomer) is hypothesized to be estrogenic when present in sufficient quantities in the body, raising concerns that widespread exposure to BPA may impact human health. To better understand the internal exposure of adult humans to BPA and the relationship between the serum and urinary pharmacokinetics of BPA, a clinical exposure study was conducted. Blood and urine samples were collected approximately hourly over a 24-h period from 20 adult volunteers who ingested 100% of one of three specified meals comprising standard grocery store food items for breakfast, lunch, and dinner. The volunteers' average consumption of BPA, estimated from the urinary excretion of total BPA ((TOT)BPA = conjugated BPA + BPA), was 0.27 mu g/kg body weight (range, 0.03-0.86), 21% greater than the 95th percentile of aggregate exposure in the adult U.S. population. A serum time course of (TOT)BPA was observable only in individuals with exposures 1.3-3.9 times higher than the 95th percentile of aggregate U.S. exposure. The (TOT)BPA urine concentration T-max was 2.75 h (range, 0.75-5.75 h) post-meal, lagging the serum concentration T-max by similar to 1 h. Serum (TOT)BPA area under the curve per unit BPA exposure was between 21.5 and 79.0 nM center dot h center dot kg/mu g BPA. Serum (TOT)BPA concentrations ranged from less than or equal to limit of detection (LOD, 1.3 nM) to 5.7 nM and were, on average, 42 times lower than urine concentrations. During these high dietary exposures, (TOT)BPA concentrations in serum were undetectable in 83% of the 320 samples collected and BPA concentrations were determined to be less than or equal to LOD in all samples.