Fluorescence turn-on detection of iodide, iodate and total iodine using fluorescein-5-isothiocyanate-modified gold nanoparticles
Fluorescence turn-on detection of iodide, iodate and total iodine using fluorescein-5-isothiocyanate-modified gold nanoparticles
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DOI：
10.1039/b905426j
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发表时间：
2009-01-01
期刊：
影响因子：
4.2
通讯作者：
Tseng, Wei-Lung
中科院分区：
文献类型：
--
作者：
Chen, Yi-Ming;Cheng, Tian-Lu;Tseng, Wei-Lung
使用异硫氰酸荧光素修饰的金纳米颗粒（FITC-AuNPs）实现了I-的选择性开启荧光检测。FITC分子，在碱性溶液中发出强烈的荧光，当它们通过异硫氰酸酯基团附着在金纳米颗粒表面时，会被严重淬灭。当加入I-时，由于I-吸附在AuNPs表面，FITC分子被分离。结果，释放的FITC分子恢复到其原始荧光强度。由于I-对Au表面的结合亲和力高于Br-、Cl-或F-，因此FITC-AuNP对I-的选择性明显高于对这些其他阴离子的选择性。同时，用抗坏血酸将IO 3-还原为I-后，利用FITC-AuNPs成功实现了IO 3-的检测。在最佳pH和AuNP浓度下，使用该探针的I-和IO 3-的最低可检测浓度分别为10.0和50.0 nM。FITC-AuNP提供了许多优点，包括易于制备、选择性、灵敏度和低成本。将该探针应用于食盐和海水中总碘的测定。
Selective turn-on fluorescence detection of I- was accomplished using fluorescein isothiocyanate-decorated gold nanoparticles (FITC-AuNPs). FITC molecules, which fluoresce strongly in an alkaline solution, were severely quenched when they were attached to the surface of AuNPs through their isothiocyanate group. Upon the addition of I-, FITC molecules were detached because of I- adsorption on the surface of AuNPs. As a result, released FITC molecules were restored to their original fluorescence intensity. Because I- has a higher binding affinity to the surface of Au than do Br-, Cl-, or F-, the FITC-AuNPs obviously have a higher selectivity toward I- than toward these other anions. Meanwhile, after IO3- was reduced to I- with ascorbic acid, the detection of IO3- was successfully achieved using the FITC-AuNPs. Under an optimum pH and AuNP concentration, the lowest detectable concentrations of I- and IO3- using this probe were 10.0 and 50.0 nM, respectively. The FITC-AuNPs provide a number of advantages, including easy preparation, selectivity, sensitivity, and low cost. This unique probe was applied to an analysis of the total iodine in edible salt and seawater.