Rapid visualization of protein bands in preparative SDS-polyacrylamide gels.
Rapid visualization of protein bands in preparative SDS-polyacrylamide gels.
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DOI：
10.1016/s0003-2697(79)80148-7
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发表时间：
1979
影响因子：
2.9
通讯作者：
Ratchford C. Higgins;M. Dahmus
中科院分区：
文献类型：
--
作者：
Ratchford C. Higgins;M. Dahmus
一种快速灵敏的方法已被开发用于检测十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶中的蛋白条带，该方法适用于在制备规模上回收单个多肽。该过程是可逆的，并允许从制备凝胶中完全回收未修饰的蛋白质。可视化快速，需要30至40分钟，并且灵敏，检测到0.1 μg/mm 3的蛋白质。该方法使用高浓度的乙酸钠。
A rapid sensitive method has been developed for the detection of protein bands in sodiumdodecyl sulfate (SDS)-polyacrylamide gels that is suitable for the recovery of individual polypeptides on a preparative scale. The process is reversible and allows for the complete recovery of unmodified protein from preparative gels. The visualization is rapid, requiring between 30 and 40 min, and sensitive, detecting as little protein as 0.1 μg/mm3. The procedure employs high concentrations of sodium acetate.