Magnetophoretic Behavior of 3T3 Cells Incubated with Saccharide-Coated MNPs
Magnetophoretic Behavior of 3T3 Cells Incubated with Saccharide-Coated MNPs
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DOI：
10.1557/adv.2016.648
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发表时间：
2017-01-01
期刊：
影响因子：
0.8
通讯作者：
Webb, Simon J.
中科院分区：
文献类型：
--
作者：
Fallows, Thomas W.;Coxon, Thomas P.;Webb, Simon J.
已经发现提供具有糖涂层的磁铁矿纳米颗粒显著增加了纳米颗粒与细胞的相互作用。采用葡萄糖（Glc）或N乙酰葡萄糖胺（GlcNAc）包裹的磁性纳米颗粒（MNP）磁性标记3 T3成纤维细胞，研究标记细胞对外磁场的反应。发现与未涂覆的纳米颗粒一起孵育的细胞相比，与Glc或GlcNAc涂覆的纳米颗粒一起孵育的细胞更有可能朝向外部磁体移动。此外，悬浮液中的细胞比与聚苯乙烯孔板表面接触的细胞移动得快得多，更强的磁体增加了移动速度。粘附在细胞培养孔的底板上并且不在x-y平面中移动的细胞仍然可以通过围绕细胞移动外部磁体而围绕z轴旋转。
Providing magnetite nanoparticles with saccharide coatings has been found to significantly increase the interactions of the nanoparticles with cells. Glucose (Glc) or Nacetylglucosamine (GlcNAc) coated magnetic nanoparticles (MNPs) were used to magnetically label 3T3 fibroblast cells, and the response of the labelled cells to external magnetic fields was studied. It was found that cells incubated with Glc-or GlcNAc-coated nanoparticles were much more likely to move towards an external magnet than those incubated with uncoated nanoparticles. Furthermore, cells in suspension moved much faster than those in contact with the surface of polystyrene well plates, with stronger magnets increasing the speed of movement. Cells that were adhering to the floor of the cell culture well and did not move in the x-y plane could still be rotated about the z-axis by moving the external magnet around the cell.