Comparative study on the distribution patterns of P2X1–P2X6 receptor immunoreactivity in the brainstem of the rat and the common marmoset (Callithrix jacchus): Association with catecholamine cell groups
Comparative study on the distribution patterns of P2X1–P2X6 receptor immunoreactivity in the brainstem of the rat and the common marmoset (Callithrix jacchus): Association with catecholamine cell groups
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DOI：
10.1002/1096-9861(20001127)427:4
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发表时间：
2000-11
影响因子：
2.5
通讯作者：
S. Yao;J. Barden;D. Finkelstein;M. Bennett;A. Lawrence
中科院分区：
文献类型：
--
作者：
S. Yao;J. Barden;D. Finkelstein;M. Bennett;A. Lawrence
本研究采用免疫组织化学方法研究了P2 X1-P2 X6受体亚型在大鼠和普通绒猴后脑的分布。此外，使用双标记免疫荧光来确定儿茶酚胺细胞组与各种P2 X受体之间的共定位程度。数据表明，尽管物种、脑核和P2 X受体亚型之间存在差异，但所有六种P2 X受体在大鼠后脑和绒猴后脑中都存在广泛的分布模式。大鼠孤束核（NTS）、前庭内侧核、臂旁内侧核和臂旁外侧核内均可见P2 X受体的强阳性染色。在舌下神经核、楔状核、下橄榄核、舌下前置肌、延髓头端腹外侧（RVLM）和蓝斑中观察到中度染色。在薄束核、三叉神经中脑核和脑桥中央灰质中也观察到染色。在绒猴中，显著的P2 X受体样免疫反应性发生在NTS、内侧楔束核、舌下前置肌和内侧前庭核。在最后区、迷走神经背侧运动核、外侧楔状核、外侧网状核、三叉神经脊束核、RVLM和下橄榄核中观察到中度染色。对含有酪氨酸羟化酶（TH）的细胞的免疫荧光双标记表明，P2 X受体的所有亚型均表现出一定程度的与TH共定位。TH和P2 X受体双标记的最高比例在A5区域（具有P2 X2亚基），而双标记细胞的最低比例发生在NTS的C2区域的P2 X5亚基。这些发现支持细胞外腺苷5′-三磷酸在脑干内快速突触神经传递中的作用。神经学比较杂志427：485-507，2000.© 2000 Wiley利斯公司
The present study investigated the topographical distribution of P2X1–P2X6 receptor subtypes in the rat and common marmoset hindbrain by immunohistochemistry. In addition, double‐labeling immunofluorescence was used to determine the extent of colocalization between catecholamine cell groups and the various P2X receptors. The data demonstrate a widespread distribution pattern for all six P2X receptors throughout both the rat hindbrain and the marmoset hindbrain, although distinctions between species, brain nuclei, and P2X receptor subtypes exist. In rat, dense staining for the P2X receptors was found in the nucleus of the solitary tract (NTS), medial vestibular nucleus, and medial and lateral parabrachial nuclei. Moderate staining was observed in the hypoglossal nucleus, cuneate nucleus, inferior olive, prepositus hypoglossi, rostral ventrolateral medulla (RVLM), and locus coeruleus. Staining was also observed in the gracile nucleus, the mesencephalic trigeminal nucleus, and the central pontine gray. In marmoset, prominent P2X receptor‐like immunoreactivity occurred in the NTS, medial cuneate nucleus, prepositus hypoglossi, and medial vestibular nucleus. Moderate staining was observed in the area postrema, dorsal motor nucleus of the vagus, lateral cuneate, lateral reticular, spinal trigeminal nucleus, RVLM, and inferior olive. Immunofluorescent double labeling of tyrosine hydroxylase (TH)‐containing cells revealed that all subtypes of P2X receptors show some degree of colocalization with TH. The highest proportion of TH and P2X receptor double labeling was in the A5 region (with the P2X2 subunit), whereas the lowest proportion of double‐labeled cells occurred in the C2 region of the NTS for the P2X5 subunit. These findings support a role for extracellular adenosine 5′‐triphosphate in fast synaptic neurotransmission within the brainstem. J. Comp. Neurol. 427:485–507, 2000. © 2000 Wiley‐Liss, Inc.