Trigeminal PAD as disynaptically evoked by stimulation of the trigeminal sensory branches.
Trigeminal PAD as disynaptically evoked by stimulation of the trigeminal sensory branches.
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三叉神经 PAD 由三叉神经感觉分支刺激引起。
DOI：
10.1016/0006-8993(74)90871-3
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发表时间：
1974
期刊：
影响因子：
2.9
通讯作者：
S. Ishimine
中科院分区：
文献类型：
--
作者：
Y. Nakamura;T. Murakami;S. Ishimine
初级传入去极化（PAD）的时间过程已确定在脊髓4，5和楔状核1轴突内记录。据报道，脊髓中外周诱发的PAD的中枢潜伏期通常为2.0-3.0 msec，并且假定该PAD的中间神经元链由至少两个串联顺序为z的中间神经元组成。在楔状核中，从传入齐射的最快成分到达楔状核到PAD发作的最短潜伏期为2.0-2.2 msec，并且PAD被假定为通过至少两个突触诱导1。在三叉神经系统中，迄今为止尚未报道PAD的轴突内研究，并且仅使用Wall技术间接跟踪三叉神经PAD的时间过程2，6。作为三叉神经PAD中枢通路研究的第一步，我们尝试用Wall技术和轴索内记录方法测定三叉神经周围诱发PAD的中枢潜伏期。本报告将描述这些结果表明，三叉神经PAD可以诱发通过一个中间神经元的三叉神经感觉支的刺激。实验在用戊巴比妥钠（最初35 mg/kg ip）麻醉并用三乙基碘化没食子胺固定的猫上进行。右侧额神经（FR）、眶下神经（IO）和舌神经（LING）用于刺激和记录。将这些神经与周围组织分离，沿外周切片并安装在双极银丝电极上。PAD的时程通过Wall技术间接检测，也通过轴突内记录直接检测。前者在极间亚核水平将双极同心电极插入右侧三叉神经脊束核，对三叉神经初级传入纤维的突触前末梢施加测试电击，记录FR、IO和LING的逆向诱发电位。将条件性电击施加于FR、IO和LING中的一种。对于轴突内
The time course of primary afferent depolarization (PAD) has been determined by intra-axonal recording in the spinal cord4, 5 and the cuneate nucleus 1. The central latency of the peripherally evoked PAD in the spinal cord was reported to be usually 2.0-3.0 msec and the interneuronal chain for this PAD was assumed to consist of at least two interneurons in serial order z. In the cuneate nucleus the shortest latency from the arrival of the fastest component of the afferent volley at the cuneate nucleus to the onset of the PAD was shown to be 2.0-2.2 msec and the PAD was postulated to be induced via at least two synapses 1. In the trigeminal system, no intra-axonal study of the PAD has so far been reported and the time course of the trigeminal PAD has been followed only indirectly using Wall's technique2, 6. As the first step of the study of the central pathways for the trigeminal PAD, we tried to determine the central latency of the peripherally evoked PAD in the trigeminal nerve not only by Wall's technique 7 but also by an intra-axonal recording method. This report will describe those results which indicate that the trigeminal PAD can be evoked via one interneuron by stimulation of the trigeminal sensory branches. Experiments were performed on cats anesthetized with pentobarbital sodium (35 mg/kg ip initially) and immobilized with gallamine triethiodide. The frontal (FR), infraorbital (IO) and lingual nerves (LING) on the right side were used for stimulation and recording. These nerves were separated from the surrounding tissue, sectioned peripherally and mounted on bipolar silver wire electrodes. The time course of PAD was examined indirectly by Wall's technique as well as directly by intra-axonal recording. In the former, a bipolar concentric electrode was inserted into the trigeminal spinal nucleus on the right side at the level of the subnucleus interpolaris to apply test shocks to the presynaptic terminals of the trigeminal primary afferent fibers, and the antidromically evoked potentials were recorded from FR, IO and LING. The conditioning shocks were applied to one of FR, IO and LING. For intra-axonal