Utility of DNA Microarrays for detection of viruses in acute respiratory tract infections in children
Utility of DNA Microarrays for detection of viruses in acute respiratory tract infections in children
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DOI：
10.1016/j.jpeds.2007.12.035
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发表时间：
2008-07-01
影响因子：
5.1
通讯作者：
Ganem, Don
中科院分区：
文献类型：
--
作者：
Chiu, Charles Y.;Urisman, Anatoly;Ganem, Don
目的评价泛病毒基因芯片平台的实用性研究设计将病毒芯片与常规的直接荧光抗体（DFA）和聚合酶链反应（PCR）进行比较，方法：应用病毒芯片技术对222名儿童的278份鼻咽分泌物标本进行呼吸道病毒检测。在性能上上级DFA，在标准DFA板中包括的7种呼吸道病毒的检测中显示出19%的增加，并且与病毒特异性PCR相似（敏感性，85%至90%;特异性，>= 99%;阳性预测值，94%至96%;阴性预测值，97%至98%），用于检测呼吸道合胞病毒、甲型流感病毒和鼻病毒/肠道病毒。病毒芯片，也检测到病毒不定期测试或错过了DFA和PCR，以及双重感染和感染的危重病人，DFA未能detect.Conclusions鉴于其有利的灵敏度和特异性的配置文件和扩展的检测谱，基于芯片的病毒检测有望为儿科RTI的临床诊断。
Objective To assess the utility of a panviral DNA microarray platform (Virochip) in the detection of viruses associated with pediatric respiratory tract infections (RTIs).Study design The Virochip was compared with conventional direct fluorescent antibody (DFA)- and polymerase chain reaction (PCR)-based testing for the detection of respiratory viruses in 278 consecutive nasopharyngeal aspirate samples from 222 children.Results The Virochip was superior in performance to DFA, showing a 19% increase in the detection of 7 respiratory viruses included in standard DFA panels, and was similar to virus-specific PCR (sensitivity, 85% to 90%; specificity, >= 99%; positive predictive value, 94% to 96%; negative predictive value, 97% to 98%) in the detection of respiratory syncytial virus, influenza A, and rhinoviruses/enteroviruses. The Virochip, also detected viruses not routinely tested for or missed by DFA and PCR, as well as double infections and infections in critically ill patients that DFA failed to detect.Conclusions Given its favorable sensitivity and specificity profile and expanded spectrum for detection, microarray-based viral testing holds promise for clinical diagnosis of pediatric RTIs.