Silk-elastinlike protein polymer hydrogels: Influence of monomer sequence on physicochemical properties
Silk-elastinlike protein polymer hydrogels: Influence of monomer sequence on physicochemical properties
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DOI：
10.1016/j.polymer.2008.11.047
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发表时间：
2009-01-16
期刊：
影响因子：
4.6
通讯作者：
Ghandehari, Hamidreza
中科院分区：
文献类型：
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作者：
Dandu, Ramesh;Von Cresce, Arthur;Ghandehari, Hamidreza
与现有的水凝胶形成类似物（SELP-415 K和SELP-47 K）相比，具有8个丝和15个弹性蛋白单元以及赖氨酸（K）修饰的弹性蛋白的丝-弹性蛋白样蛋白聚合物SELP-815 K被遗传工程化为具有更长的丝和弹性蛋白单元。三个SELP（具有相似的MW）的水凝胶的结构-功能关系进行了研究。结果表明，这些水凝胶的平衡溶胀比是聚合物结构、浓度、固化时间和介质离子强度的函数。溶胀不受pH值变化的影响。通过动态力学分析观察到的储能模量和从小角中子散射获得的Debye-Bueche相关长度提供了结构洞察力，表明这些水凝胶中的交联密度遵循SELP-47 K> SELP-815 K> SELP-415 K的顺序。这些结果暗示了弹性蛋白块的长度在控制交联水凝胶网络的间距中的重要性，以及丝在控制其三维结构的刚度中的重要性。(C)2008爱思唯尔有限公司保留所有权利。
Silk-elastinlike protein polymer, SELP-815K, with eight silk and fifteen elastin units and a lysine (K) modified elastin, was genetically engineered with longer silk and elastin units compared to existing hydrogel forming analogs (SELP-415K and SELP-47K). Hydrogels of the three SELPs (with similar MWs) were investigated for their structure-function relationships. Results indicate that equilibrium swelling ratio in these hydrogels is a function of polymer structure, concentration, cure time and ionic strength of media. Swelling was not influenced by the changes in pH. Storage moduli observed by dynamic mechanical analysis and the Debye-Bueche correlation length obtained from small-angle neutron scattering provided structural insight that suggests the cross-linking densities in these hydrogels follow the order SELP-47K > SELP-815K > SELP-415K. These results allude to the importance of the length of elastin blocks in governing the spacing of the cross-linked hydrogel network and that of silk in governing the stiffness of their 3-dimensional structures. (C) 2008 Elsevier Ltd. All rights reserved.