Properties of alternative microbial hosts used in synthetic biology: towards the design of a modular chassis.
Properties of alternative microbial hosts used in synthetic biology: towards the design of a modular chassis.
复制标题
合成生物学中使用的替代微生物宿主的特性：朝着模块化底盘设计。
DOI：
10.1042/ebc20160015
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发表时间：
2016-11-30
影响因子：
6.4
通讯作者：
Jiménez JI
中科院分区：
文献类型：
--
作者：
Kim J;Salvador M;Saunders E;González J;Avignone-Rossa C;Jiménez JI
底盘是在合成生物学中用作工程生物系统的受体的细胞宿主。它们需要传播遗传信息并表达其中编码的基因。尽管底盘是遗传电路适当功能的基本要素，但在设计阶段很少考虑底盘。因此，电路被转移到实验室中常用的模型生物体，如大肠杆菌，这可能是所需功能的次优。在这篇综述中，我们讨论了一些理想的性能在一个多功能的底盘，并总结了一些例子的替代主机合成生物学适合工程。这些属性包括一个合适的生活方式，一个强大的细胞壁，良好的知识，其监管网络以及与外源电路的主机组件的相互作用，和开发全细胞模型和可调的代谢通量，可以允许更好地分配细胞资源（代谢物，ATP，核苷酸，氨基酸，转录和翻译机器）的可能性。我们强调恶臭假单胞菌，广泛用于许多不同的生物技术应用作为合成生物学的一个突出的有机体，由于其代谢多样性，鲁棒性和易于操作。
The chassis is the cellular host used as a recipient of engineered biological systems in synthetic biology. They are required to propagate the genetic information and to express the genes encoded in it. Despite being an essential element for the appropriate function of genetic circuits, the chassis is rarely considered in their design phase. Consequently, the circuits are transferred to model organisms commonly used in the laboratory, such as Escherichia coli, that may be suboptimal for a required function. In this review, we discuss some of the properties desirable in a versatile chassis and summarize some examples of alternative hosts for synthetic biology amenable for engineering. These properties include a suitable life style, a robust cell wall, good knowledge of its regulatory network as well as of the interplay of the host components with the exogenous circuits, and the possibility of developing whole-cell models and tuneable metabolic fluxes that could allow a better distribution of cellular resources (metabolites, ATP, nucleotides, amino acids, transcriptional and translational machinery). We highlight Pseudomonas putida, widely used in many different biotechnological applications as a prominent organism for synthetic biology due to its metabolic diversity, robustness and ease of manipulation.