Age dependent apoptosis and loss of rhabdosphincter cells
Age dependent apoptosis and loss of rhabdosphincter cells
复制标题
DOI：
10.1016/s0022-5347(05)67106-6
复制
发表时间：
2000-11-01
影响因子：
6.6
通讯作者：
Konwalinka, G
中科院分区：
文献类型：
--
作者：
Strasser, H;Tiefenthaler, M;Konwalinka, G
目的：据我们所知，括约肌机制与年龄相关的形态和功能的确切变化尚未被研究。因此，对不同年龄段的人的尿路和横纹括约肌的细胞密度和细胞凋亡情况进行检测，以探讨这些结构在衰老过程中的变化。材料和方法：标本取自5周至92岁的16具男性和7名女性身体。组织学切片取自横纹括约肌和尿路的3个不同平面。采用末端脱氧核苷酸转移酶介导的脱氧尿嘧啶核苷缺口末端标记法检测大鼠尿路和横纹括约肌的细胞凋亡。结果：横纹肌纤维的凋亡率随年龄的增加而增加，横纹肌细胞数随年龄的增加而减少。5周龄新生儿横纹肌占87.6%，1岁女性横纹肌占34.2%。总体而言，标本的年龄与横纹肌细胞的体积密度下降之间存在明显的线性关系。结论：老年人横纹括约肌中由于细胞凋亡而导致的横纹肌细胞数量的急剧减少是该人群压力性尿失禁高发的形态学基础。
Purpose: To our knowledge the exact age dependent morphological and functional changes of the sphincter mechanism have not been investigated. Therefore, cell densities of the urethra and the urethral rhabdosphincter across various age groups, and the appearance of apoptosis were examined to explore the changes in these structures during the aging process.Materials and Methods: Specimens were obtained from 16 male and 7 female cadavers 5 weeks to 92 years old. Histological sections were taken from 3 different levels of the rhabdosphincter and urethra. The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling method was used to detect apoptosis in the urethra and rhabdosphincter. In all specimens relative Volume densities of the striated muscle fibers, apoptotic indexes and diameters of the rhabdosphincter and urethra were determined.Results: An age dependent increase of apoptosis of the striated muscle fibers of the rhabdosphincter led to a dramatic decrease in the number of striated muscle cells. In the 5-week-old neonate 87.6% and in the gl-year-old woman 34.2% of the rhabdosphincter consisted of striated muscle cells. Overall, a direct linear correlation between the age of the specimens and decrease in volume densities of the striated muscle cells was evident.Conclusions: The dramatic decrease in the number of striated muscle cells in the rhabdosphincter of the elderly due to apoptosis represents the morphological basis for the high incidence of stress incontinence in this population.