Deep proteomics of mouse skeletal muscle enables quantitation of protein isoforms, metabolic pathways, and transcription factors.
Deep proteomics of mouse skeletal muscle enables quantitation of protein isoforms, metabolic pathways, and transcription factors.
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DOI：
10.1074/mcp.m114.044222
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发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
Mann M
中科院分区：
文献类型：
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作者：
Deshmukh AS;Murgia M;Nagaraj N;Treebak JT;Cox J;Mann M
骨骼肌占个体体重的40%，在运动和全身代谢中起着至关重要的作用。骨骼肌蛋白质组学研究具有挑战性，因为大量的收缩蛋白会干扰调节蛋白的检测。使用最先进的MS工作流程和将C2C12细胞系模型的鉴定映射到组织的策略，我们共鉴定了10，218种蛋白质，包括骨骼肌特异性转录因子如myod1和肌细胞生成素以及昼夜节律钟蛋白。我们获得了肌肉细胞系和骨骼肌中表达的蛋白质的绝对丰度，这应该是一个有价值的资源。葡萄糖摄取信号传导途径和葡萄糖和脂质代谢途径中的蛋白质亚型的定量提供了与组织相比的细胞系的详细代谢图。这揭示了在酶同工型水平上，肌肉组织中AMP活化蛋白激酶和胰岛素信号传导的意外复杂调节。
Skeletal muscle constitutes 40% of individual body mass and plays vital roles in locomotion and whole-body metabolism. Proteomics of skeletal muscle is challenging because of highly abundant contractile proteins that interfere with detection of regulatory proteins. Using a state-of-the art MS workflow and a strategy to map identifications from the C2C12 cell line model to tissues, we identified a total of 10,218 proteins, including skeletal muscle specific transcription factors like myod1 and myogenin and circadian clock proteins. We obtain absolute abundances for proteins expressed in a muscle cell line and skeletal muscle, which should serve as a valuable resource. Quantitation of protein isoforms of glucose uptake signaling pathways and in glucose and lipid metabolic pathways provides a detailed metabolic map of the cell line compared with tissue. This revealed unexpectedly complex regulation of AMP-activated protein kinase and insulin signaling in muscle tissue at the level of enzyme isoforms.