Cola nitida infusion modulates cardiometabolic activities linked to cardiomyopathy in diabetic rats
Cola nitida infusion modulates cardiometabolic activities linked to cardiomyopathy in diabetic rats
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DOI：
10.1016/j.fct.2021.112335
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发表时间：
2021-06-15
影响因子：
4.3
通讯作者：
Islam, Md Shahidul
中科院分区：
文献类型：
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作者：
Erukainure, Ochuko L.;Msomi, Nontokozo Z.;Islam, Md Shahidul
本研究探讨了可乐籽对糖尿病大鼠心脏糖尿病心肌病的治疗机制。 2 型糖尿病 (T2D) 大鼠接受 150 或 300 mg/kg 体重 (bw) 的 C.nitida 输注治疗。治疗六周后处死大鼠，并收获心脏。谷胱甘肽、超氧化物歧化酶和过氧化氢酶活性水平下降，丙二醛水平升高，表明 T2D 诱导时氧化应激激增。 T2D 大鼠心脏中乙酰胆碱酯酶和 ATP 酶的活性显着升高，ENTPDase 和 5' 核苷酸酶活性受到抑制，表现出胆碱能和嘌呤能功能障碍。 T2D 的诱导进一步导致 ACE 活性升高并改变心肌形态。用 C.nitida 输注治疗导致这些生物标志物的活性和水平逆转，同时保持完整的形态。输注导致脂肪酶活性降低和糖尿病产生的心脏脂质代谢物的消耗，同时产生饱和和不饱和脂肪酸、脂肪酯和醇。在用 C.nitida 输注治疗的 T2D 大鼠中，缩醛磷脂合成和长链饱和脂肪酸途径的线粒体 β 氧化也出现失活。这些结果表明金针花输注液对糖尿病心肌病的治疗作用。
This study investigated the therapeutic mechanism of Cola nitida seeds on diabetic cardiomyopathy in hearts of diabetic rats. Type 2 diabetic (T2D) rats were treated with C. nitida infusion at 150 or 300 mg/kg body weight (bw). The rats were sacrificed after 6 weeks of treatment, and their hearts harvested. There was an upsurge in oxidative stress on induction of T2D as depicted by the depleted levels of glutathione, superoxide dismutase and catalase activities, and elevated malondialdehyde level. The activities of acetylcholinesterase, and ATPase were significantly elevated, with suppressed ENTPDase and 5'nucleotodase activities in hearts of T2D rats depicting cholinergic and purinergic dysfunctions. Induction of T2D further led to elevated activity of ACE and altered myocardial morphology. Treatment with C. nitida infusion led to reversal of these biomarkers' activities and levels, while maintaining an intact morphology. The infusion caused decreased lipase activity and depletion of diabetes-generated cardiac lipid metabolites, while concomitantly generating saturated and unsaturated fatty acids, fatty esters and alcohols. There was also an inactivation of plasmalogen synthesis and mitochondrial betaoxidation of long chain saturated fatty acids pathways in T2D rats treated with C. nitida infusion. These results indicate the therapeutic effect of C. nitida infusion against diabetic cardiomyopathy.