Effects of maternal exposure to di-isononylphthalate (DINP) and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p′-DDE) on steroidogenesis in the fetal rat testis and adrenal gland
Effects of maternal exposure to di-isononylphthalate (DINP) and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p′-DDE) on steroidogenesis in the fetal rat testis and adrenal gland
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DOI：
10.1016/j.reprotox.2009.03.002
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发表时间：
2009-07-01
影响因子：
3.3
通讯作者：
Toppari, J.
中科院分区：
文献类型：
--
作者：
Adamsson, A.;Salonen, V.;Toppari, J.
在关键的发育窗口（即性别分化）期间接触抗雄激素可以使男性表型永久去男性化。在这里，我们研究了发育暴露于邻苯二甲酸二异壬酯（DINP）（2·50和750 mg/kg）和1,1-二氯-2,2-双（对氯苯基）乙烯（p,p'-DDE）（50和100 mg/kg）对19.5日龄胎儿Sprague-Dawley大鼠睾丸和肾上腺类固醇生成的影响。母亲在胚胎日 (ED) 13.5-17.5 时接触 DINP 或 p,p'-DDE 不会下调 ED 19.5 雄性大鼠胎儿的类固醇生成活性。睾丸和肾上腺StAR、P450scc、3β-HSD和雄激素受体（AR）的蛋白表达水平没有显示任何变化。然而，p,p'-DDE 导致 ED 19.5 大鼠睾丸和肾上腺中类固醇生成细胞的超微结构明显异常。这些结构改变会扰乱胎儿睾丸和肾上腺的发育和功能，这可能会在以后的生活中变得明显。 (C) 2009 Elsevier Inc. 保留所有权利。
Exposure to antiandrogens during the critical developmental window (i.e. sexual differentiation) can permanently demasculinize the male phenotype. Here we have investigated the effects of developmental exposure to di-isononylphthalate (DINP) (2 50 and 750 mg/kg) and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p'-DDE)(50 and 100 mg/kg) on 19.5-day-old fetal Sprague-Dawley rat testicular and adrenal steroidogenesis. Maternal exposure to DINP or p,p'-DDE on embryonic days (EDs) 13.5-17.5 did not down-regulate the activity of steroidogenesis in ED 19.5 male rat fetus. Protein expression levels of testicular and adrenal StAR, P450scc, 3 beta-HSD and androgen receptor (AR) did not show any changes. However, p,p'-DDE caused clear abnormalities in the ultrastructure of steroidogenic cells in ED 19.5 rat testis and adrenal. These structural alterations can disturb the development and function of fetal testis and adrenal that may become evident later in life. (C) 2009 Elsevier Inc. All rights reserved.