Identification of Bacillus subtilis σW-dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli
Identification of Bacillus subtilis σW-dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli
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DOI：
10.1111/j.1365-2958.2006.05131.x
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发表时间：
2006-05-01
影响因子：
3.6
通讯作者：
Helmann, JD
中科院分区：
文献类型：
--
作者：
Butcher, BG;Helmann, JD
枯草芽孢杆菌产生许多不同结构和特异性的抗生素。在这里，我们确定了sigma(W)，一种胞质外功能（ECF） sigma因子，在提供对其他芽孢杆菌产生的抗菌化合物的内在抗性方面的突出作用。通过使用一组已知的30个sigma(W)依赖操纵子的枯草芽孢杆菌突变体，我们确定了至少三种不同抗菌化合物的抗性基因。ydbST和fosB基因对解淀粉芽孢杆菌FZB42产生的抗菌化合物具有抗性，yqeZ-yqfAB操纵子对SP β噬菌体编码的细菌素亚blancin具有抗性，yknWXYZ操纵子和yfhL对抗菌肽SdpC具有抗性。YfhL编码SdpI的一个平行序列，这是一种提供对SdpC免疫的膜蛋白。在竞争实验中，我们发现sigma(W)是枯草芽孢杆菌抵抗抗生素杀死和竞争菌株入侵的关键因素。加上之前的观察，sigma(W)提供了对链霉菌抗生素磷霉素的诱导抗性，这些研究支持了sigma(W)控制土壤细菌微生物生态中重要的抗生素调控的观点。
Bacillus subtilis produces many antibiotics of varying structures and specificity. Here we identify a prominent role for sigma(W), an extracytoplasmic function (ECF) sigma factor, in providing intrinsic resistance to antimicrobial compounds produced by other Bacilli. By using a panel of B. subtilis mutants disrupted for each of the 30 known sigma(W)-dependent operons we identified resistance genes for at least three different antimicrobial compounds. The ydbST and fosB genes contribute to resistance to antimicrobial compound(s) produced by B. amyloliquefaciens FZB42, the yqeZ-yqfAB operon provides resistance to the SP beta prophage-encoded bacteriocin sublancin, and the yknWXYZ operon and yfhL provide resistance to the antimicrobial peptide SdpC. YfhL encodes a paralogue of SdpI, a membrane protein that provides immunity to SdpC. In competition experiments, we identify sigma(W) as a key factor in allowing B. subtilis to resist antibiotic killing and encroachment by competing strains. Together with the previous observation that sigma(W) provides inducible resistance against the Streptomyces antibiotic fosfomycin, these studies support the notion that sigma(W) controls an antibiosis regulon important in the microbial ecology of soil bacteria.